here - First Legume Society Conference (LSC1)

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Deploying genomics tools for improving resistance to leaf rust through marker- assisted
backcrossing in cultivated groundnut (Arachis hypogaea L.)
Manish K Pandey 1 , Janila Pasupuleti 1 , Shyam N Nigam 1 , Manda Sriswathi 1 , Makanahally V
Chennabyre Gowda 2 , P Nagesh 1 , Pawan Khera 1 , Rajeev K Varshney 1,3
1 International Crops Research Institute for the Semi-Arid Tropics, Hyderabad, India
2 University of Agricultural Sciences, Dharwad, India
3 CGIAR Generation Challenge Programme, CIMMYT, Mexico DF, Mexico
Rust (Puccinia arachidis), is a major devastating fungal disease of groundnut (Arachis hypogaea) in
semi-arid tropics regions of the world resulting in significant yield loss. It has been witnessed
recently that integration of genomics tools with conventional breeding leads development of
improved cultivars in less time and with increased efficiency. However, availability of genomics
tools such as validated tightly linked markers to traits of interest is prerequisite to improve
resistance in cultivars through marker-assisted breeding. In this context, a major QTL flanking
with four SSR markers (IPAHM103, GM2079, GM2301, GM1536) for rust resistance
contributing phenotypic variance up to 82.62% has been identified in TAG24 x GPBD4.This
QTL and associated markers were validated in a wide range of germplasm and also including
another RIL mapping population (TG26 x GPBD4). Then subsequently, this QTL for rust
resistance was introgressed from GPBD 4 into three elite groundnut varieties (ICGV91114, JL24,
TAG24) from GPBD 4 through marker-assisted backcrossing (MABC) approach. Among the
four SSR markers, IPAHM103 behaves as a dominant marker while the remaining three markers
were co-dominant in nature and hence, we deployed them in selection of
heterozygotes/homozygotes among segregating backcrossed progenies. In summary, a total of
200 introgression lines (117 progenies of BC2F5 and 83 of BC3F4) with homozygous resistance
allele were developed and evaluated. These introgression lines have shown par resistance than
donor, GPBD4, for rust resistance and other agronomic traits. The promising lines with
improved rust resistance and desirable yield will be further subjected to multilocational trials for
varietal release.
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