here - First Legume Society Conference (LSC1)

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
_________________________________________________________________________________________
Mapping of single-locus and epistatic QTLs for seed morpho-agronomic traits from an
adapted x exotic nuña bean population
Fernando J Yuste-Lisbona 1 , Carmen Capel 1 , Ana M González 2 , Manuel García-Alcázar 1 , Juan
Capel 1 , Antonio M De Ron 2 , Rafael Lozano 1 , Marta Santalla 2
1 Centro de Investigación en Biotecnología Agroalimentaria (BITAL). Campus de Excelencia Internacional
Agroalimentario, CeiA3. Universidad de Almería. Almería, Spain
2 Departamento de Recursos Fitogenéticos, Grupo de Biología de Agrosistemas. Misión Biológica de Galicia-
CSIC. Pontevedra, Spain
Common bean (Phaseolus vulgaris L.) is a widely grown and important economic grain legume.
Seed quality is a critical attribute in determining commercial acceptability of common bean.
Thereby, to obtain insights into the genetics of seed quality, a population of 185 recombinant
inbred lines (RILs) derived from an Andean intra-gene pool cross, involving PMB0225 (dry bean)
and PHA1037 (nuña bean) parents, was evaluated for seven seed traits under six different
environments (long-day and short-day natural photoperiod conditions). Multi-environment QTL
analyses performed detected a total of 46 QTLs, which were mapped throughout all linkage
groups: 21 had only additive effects, 17 had only epistatic effects and eight had both additive and
epistatic effects. For seed dimension and weight traits, 19 single-locus QTLs and 13 epistatic
QTLs involved in eight epistatic interactions were detected; while for seed color and brightness,
10 single-locus QTLs and 12 epistatic QTLs involved in seven epistatic interactions were
identified. Interactions between QTLs and environment were also detected, but despite this fact,
QTLs with differential effect on long-day and short-day environments were not found. It may be
concluded that i) the genetics of seed quality is complex in nature, where not only additive effects
but also epistasis are important components of the genetic variance in the Andean common bean
background, and ii) the QTLs here reported will play a significant role in enhancing marker
assisted selection efficiency for breeding nuña beans. The work was supported by the PET2008-
0167 and AGL2011-25562 projects of the Ministerio de Economía y Competitividad from Spain.
182