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Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Results of in vitro selection of pea (Pisum sativum L.) for drought-resistance
Galina Soboleva
The All-Russia Research Institute of Legumes and Groat Crops, Orel, Russia
The purpose of researches was the development of in vitro system for selection of droughtresistant
variants of pea. Results of our experiments showed that the selective system with
polyethylene glycol (PEG-6000) in concentration of 15-20% can be used for selection of
osmotic-resistant callus lines. Ability of pea callus to regenerate shoots after subculturing on
selective media was shown. It was established, that efficiency of shoot regeneration depended on
an initial genotype and PEG concentration in selective media. There was successfully obtained
rooted pea regenerants on a nutrient medium containing auxin. Generation R 2 … R 3 obtained as
a result of selection on medium with PEG, has been studied in vegetative experiment with
artificial drought and in field trials. Ability to keep water during fading is one of the basic
parameters of drought resistance of plants. Regenerated lines, selected in rigid selective loading,
have shown the superiority over an initial cultivar on water-keeping ability. Thus the share of a
working adsorbing surface of root system increased and reduction of the stipules area was
observed. The comparative analysis of yield components has shown, that the majority of
regenerated lines have significantly exceeded an initial cultivar in many studied parameters.
Regenerated lines have appeared to be long-stem and late-ripening. Number of productive nodes
significantly depended on environmental conditions and reduced in 1,5-2 times in adverse year.
Regenerated lines had higher number of productive nodes and pods on a plant in comparison
with initial cultivars.
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