here - First Legume Society Conference (LSC1)

Recommendations

Info

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013 _________________________________________________________________________________________ Tagging Genomic Regions Controlling YMV Resistance with SSR markers in Mothbean [Vigna aconitifolia (Jaq.)] KP Viswanatha 1 , LN Yogeesh 2 , S Gangaprasad 3 1 UAS, Raichur, Karnataka, India 2 GPB, Agricultural Research Station, Hagari, UAS, Raichur, Karnataka, India 3 Departement of Genetics and Plant Breeding, Collage of Agriculture, Shimoga, UAS, Bangalore, Karnataka, India Moth bean is a drought tolerant food legume crop grown in arid & semi-arid regions of India and is an important source of dietary protein. It can be grown in low rainfall areas (200-300mm) and withstand high temperature (35-40 o C). Though it has the yield potential of 1500 kg/ha in favourable environment, the average productivity in India as low as 300-400kg/ha. Among the biotic stresses that affects crop yield diseases play a very important role in moth bean. Yellow Mosaic Virus (YMV) transmitted by whitefly (Bemisia tabaci) is most serious disease as it adversely affects the grain and fodder yield. Identification and validation of DNA markers linked to YMV resistance gene was undertaken. Parental polymorphism using 196 legume specific SSR primers combinations were carried out with resistant (MH-43) and susceptible (GMO-25) parents to identify polymorphic markers. Only 5 SSR primers were polymorphic between the parents GMO-25 and MH-43 out of 196 primers. Bulk Segregant Analysis in F 2 of the cross GMO-25(S) x MH-43 (R) using five polymorphic SSR markers, two were found polymorphic indicating putative linkage with YMV resistance gene. Linkage analysis of the 114 F 2 individuals using putatively linked SSR markers from bulk segregant analysis revealed only one PVSSR-101 marker, out of two putatively linked SSR markers with genetic distance of 20.45cM linked to resistant gene for Yellow mosaic virus disease. The PVSSR-101 marker can be used for identification and validation of homozygotes for the YMV mb resistance gene plants in early segregating population, especially during back cross breeding. 177
Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013 _________________________________________________________________________________________ Morphological and Microsatellite markers based Genetic Diversity in Cowpea [Vigna unguiculata (L.) Walp] KP Viswanatha 1 , Hasan Khan 1 , K Amitha 2 1 University of Agricultural Sciences, Raichur, Karnataka, India 2 University of Agricultural Sciences, Bangalore, Karnataka, India Morphological and molecular genetic diversity analysis on 60 selected cowpea genotypes revealed relatively low magnitude of genetic diversity at both molecular and morphological levels. Genetic similarity coefficient for all the 60 accessions ranged from 82 to 100 for morphological traits. Phenotypic based UPGMA clustering pattern separated variability among these accessions based on yield and its important attributing traits and grouped all the genotypes into seven major clusters at 92 per cent similarity coefficient. Cluster I, V and VI were solitary in nature, while cluster VII had maximum genotypes (33). Molecular analysis of the same genotypes with 42 SSR primers revealed 23 primer pairs were polymorphic and 11 were found monomorphic out of 34 primers amplified. The polymorphic primers produced allele’s ranging between 2 to 5 with an average value of 2.30. The Polymorphism Information Content ranged from 0.12 (primer CP 169) to 0.67 (primers CP 573). Based on the PIC values and number of alleles amplified, the primer CP 573 was found to be more informative in the present set of genotypes. The genetic similarity coefficient for all 60 accessions ranged from 57 to 96. UPGMA based dendrogram was obtained from the binary data deduced from the DNA profiles of the samples analyzed. Seven major clusters were formed on the basis of Jaccard’s similarity coefficient at 65 per cent. Cluster V had maximum 28 genotypes, while the cluster II was solitary in nature and indicates enormous variability in genetic constitution. A comparative study of the dendrograms based on morphological and molecular analysis revealed that SSR markers exhibiting higher range of polymorphism produced more informative bands and had more discriminating power (57-100%) than morphological diversity analysis (82-100%). 178
Page 2:
First Legume Society Conference 201
Page 5 and 6:
Scientific Committee Michael Abbert
Page 8:
Programme 9 Session 1 Achievements
Page 12 and 13:
Book of Abstracts First Legume Soci
Page 14:
Page 18 and 19:
Page 20 and 21:
Page 22 and 23:
Page 24 and 25:
Page 26 and 27:
Page 28 and 29:
Page 30 and 31:
Page 32 and 33:
Page 34 and 35:
Page 36 and 37:
Page 38 and 39:
Page 40 and 41:
Page 42 and 43:
Page 44 and 45:
Page 46:
Page 50 and 51:
Page 52 and 53:
Page 54 and 55:
Page 56 and 57:
Page 58 and 59:
Page 60 and 61:
Page 62 and 63:
Page 64 and 65:
Page 66 and 67:
Page 68:
Page 72 and 73:
Page 74 and 75:
Page 76 and 77:
Page 78 and 79:
Page 80 and 81:
Page 82 and 83:
Page 84 and 85:
Page 86 and 87:
Page 88 and 89:
Page 90 and 91:
Page 92 and 93:
Page 94 and 95:
Page 96 and 97:
Page 98 and 99:
Page 100 and 101:
Page 102 and 103:
Page 104 and 105:
Page 106 and 107:
Page 108 and 109:
Page 110 and 111:
Page 112 and 113:
Page 114:
Page 118 and 119:
Page 120 and 121:
Page 122 and 123:
Page 124 and 125:
Page 126 and 127:
Page 128 and 129: Book of Abstracts First Legume Soci
Page 138: Session 6 Translational omics for l
Page 177: Book of Abstracts First Legume Soci
Page 226: Session 8 Non-food, non-feed and ot
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
Page 235 and 236:
Page 238 and 239:
Page 240 and 241:
Page 242 and 243:
Page 244 and 245:
Page 246 and 247:
Page 248 and 249:
Page 250 and 251:
Page 252 and 253:
Page 254 and 255:
Page 256 and 257:
Page 258 and 259:
Page 260 and 261:
Page 262 and 263:
Page 264 and 265:
Page 266 and 267:
Page 268 and 269:
Page 270 and 271:
Page 272 and 273:
Page 274:
Page 278 and 279:
Page 280 and 281:
Page 282 and 283:
Page 284 and 285:
Page 286 and 287:
Page 288:
Page 292 and 293:
Page 294 and 295:
Page 296 and 297:
Page 298 and 299:
Page 300 and 301:
Page 302 and 303:
Page 304:
Page 308 and 309:
Page 310 and 311:
Page 312:
Page 315 and 316:
Name Page Name Page Capel J 182 Duc
Page 317 and 318:
Name Page Name Page Klenotičová H
Page 319 and 320:
Name Page Name Page Prusiński J 25
Page 322 and 323:
What is IFVCNS? PLATINUM SPONSOR In
Page 324 and 325:
Agromarket www.agromarket.rs Agroma
Page 326 and 327:
CID Bio-Science, Inc. www.cid-inc.c
Page 328 and 329:
Saatzucht Steinach GmbH & Co KG Don
show all

here - First Legume Society Conference (LSC1)

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?