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Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013 _________________________________________________________________________________________ Salt stress response in three pea species (Pisum arvense, P. sativum and P. fulvum) in vitro cultures Jovanka Miljuš-Djukić 1 , Nemanja Stanisavljević 1 , Svetlana Radović 2 , Živko Jovanović 1 , Aleksandar Mikić 3 , Vesna Maksimović 1 1 University of Belgrade-Institute of Molecular Genetics and Genetic Engineering, Belgrade, Serbia 2 University of Belgrade-Biological Faculty, Belgrade, Serbia 3 University of Novi Sad, Institute of Field and Vegetable Crops, Novi Sad, Serbia Salinity is one of the major abiotic factors limiting plants productivity. Processes, such as seed germination, seedling growth and vigor, vegetative growth, flowering, and fruit set, are adversely affected by high saltconcentrations, ultimately causing diminished economic yield and also quality of products. Shoots of micropropagated Pisum fulvum, P. sativum and P. arvense were treated with 75, 120, 150 and 200 mMNaClin order to study pea tissues capacity to tolerate salt stress and their antioxidant capacity. We used the phenol content and DPPH antioxidant assay as oxidative damage indicators. Results showed that salt stress caused slower plant growth, leading to an increase in lipid peroxidation and a decrease in total chlorophyll in pea tissues. Further, results showed that P. fulvum is to be considered as potentially less sensitive to high NaCl concentrations in comparison to P. sativum and P. arvense and could represents a gene pool which could be used for improving the characteristics of field pea. Acknowledgements The projects 173005 and TR-31024 of the Ministry of Education, Science and Technological Development of the Republic of Serbia and SEELEGUMES. 207
Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013 _________________________________________________________________________________________ Induced inaccessibility in pea to powdery mildew: insights gained from the use of inhibitors Juan Moral, Diego Rubiales, Elena Prats Instituto de Agricultura Sostenible, CSIC, Córdoba, Spain The term induced inaccessibility has been adopted to describe situations where early failed attacks by biotrophic pathogens to plant cells increase resistance to later attacks in that and neighboring cells. This kind of resistance widely relay in penetration resistance events however the particular cellular and molecular bases remain unknown. In the present study, we investigated the role of de novo protein formation and cytoskeleton assembly on induced inaccessibility of pea (Pisum sativum) cells. For this aim, we sequentially inoculated pea leaves of the susceptible cv. Mesire, with an inappropriate powdery mildew species (Blumeria graminisf. sp. avenae), which causes a non-host interaction and then with pea powdery mildew (Erysiphe pisi). The epidermal pea cells in which the inappropriate powdery mildew failed to penetrate and their neighboring cells showed inaccessibility to subsequent infections by the pea powdery mildew. However, when the pea plants were treated with cytochalasin, an specific inhibitor of actin polymerization and cytoesqueleton formation, or cicloheximide, a protein synthesis inhibitor, the inaccessibility were greatly repressed. Microscopic and proteomics approaches are being carried out to understand how this events influence the inaccessibility in pea. 208
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First Legume Society Conference 201
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Scientific Committee Michael Abbert
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Programme 9 Session 1 Achievements
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