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Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Strategies to identify candidate genes controlling Ascochyta blight in different legume
crops
S Ocaña-Moral 1 , E Madrid 2 , T Millán 3 , P García 4 , M Pérez de la Vega 4 , AM Torres 1
1 Área Mejora y Biotecnología, IFAPA-Centro Alameda del Obispo, Córdoba, Spain
2 Instituto de Agricultura Sostenible, CSIC, Mejora Genética Vegetal, Córdoba, Spain
3 Universidad de Córdoba, Dpto. Genética, Campus de Rabanales Edificio C5 2a planta, Córdoba, Spain
Until now complete resistance to Ascochyta blight has not been reported neither in chickpea nor
in faba bean. However, several QTLs have been located in chickpea LGs 2, 3, 4, 6 and 8. The
most significant ones are those in LGs 2 (QTL AR3) and 4 (QTL AR1 and QTL AR2). Attempts to
saturate QTL1 AR1 have been performed by developing BAC clones or by identifying candidate
genes from the model Medicago truncatula. In faba bean at least two QTLs (Af1 and Af2), assigned
to chromosomes III and II were validated across environments and generations. In a second
cross, six QTLs (Af3 to Af8), were detected. Af1 and Af3 were both assigned to chromosome III
and might correspond to the same genomic region. In this study, we are exploiting the synteny
with the model species M. truncatula and chickpea, to establish a clear homology of QTL AR1 and
Af1 in both crops based on ESTs mapping. Moreover, possible regulators revealed by
SuperSAGE analysis and associated with the ascochyta-faba bean and ascochyta-lentil
interactions are being mapped in the corresponding maps in order to identify potential targets for
molecular breeding. Finally, we will take profit from the recent chickpea whole genome sequence,
to perform wide genome comparative mapping studies with the less-studied legume crops, in
order to identify orthologous genes underlying not only blight resistance but other agronomic
important traits. Our aim is to progress from basic mapping of QTLs to fine mapping or gene
identification, thus allowing development of efficient markers for MAS in breeding.
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