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Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013 _________________________________________________________________________________________ Drought resistance studies of common bean (Phaseolus vulgaris L.): a proteomic approach Jelka Šuštar-Vozlič 1 , Tanja Zadražnik 1 , Marko Maras 1 , Kristin Hollung 2 , Wolfgang Egge- Jacobsen 3 , Vladimir Meglič 1 1Agricultural Institute of Slovenia, Ljubljana, Slovenia 2 Nofima Mat AS, Ås, Norway 3 University of Oslo, Department of Molecular Biosciences, Oslo, Norway Drought is one of the main abiotic stresses limiting common bean production worldwide; it can cause yield losses up to 80 % in some regions. The response of common bean to drought has not been widely studied, so an improved understanding of stress response mechanisms could help in developing drought resistant lines, consequently contributing to high productivity of this important food legume. A proteomic approach was used to identify drought-responsive proteins in leaves of two cultivars differing in their response to drought, Tiber and more sensitive Starozagorski čern. Total proteins from leaves of control and stressed plants were extracted and separated by 2D-DIGE. Protein spots of interest for further analysis were selected based on the analysis of variance from Progenesis SameSpot software and significant testing by PLS using the Jack-knife uncertainty test. Fifty-eight proteins whose abundance changed significantly between control and drought stressed plants were identified by LC-MS/MS in Tiber and 64 in Starozagorski čern. In order to determine which types of proteins are involved in drought stress response, the identified proteins were categorized into groups based on their putative biological functions. The majority of identified proteins were classified into functional categories that include energy metabolism, photosynthesis, ATP interconversion, protein synthesis and proteolysis, stress and defence related proteins. Interactions between identified proteins, demonstrated by bioinformatics analysis enabled a more complete insight into biological pathways and molecular functions affected by drought stress. The research provided the basic insight needed to further investigate the molecular regulatory mechanism of drought response in common bean. 175
Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013 _________________________________________________________________________________________ Contained and field experiments with GM peas in Agritec Lenka Švábová, Miroslav Griga Agritec Plant Research Ltd., Šumperk, Czech Republic Genetic transformation of pea (Pisum sativum L.) was focused on the development of increased resistance to viral and fungal pathogens and insects. For resistance to Pea Seed-borne and Pea Enation Mosaic Viruses, constructs containing sequences of respective coat proteins were used to induce pathogen-derived resistance. Starting with T1 generation, mechanical inoculation of plants with highly aggressive isolates was carried out. Plants were inspected visually (typical symptoms of virus infection) and with PCR/RT qPCR for virus presence in plant tissues. While coat protein mediated resistance (CPMR) induced resistance to PEMV expressed in T1 generation was not stable, the post-transcriptional gene silencing (PTGC) induced resistance to PSbMV was stably inherited to T3, T4 generation showing “plant recovery phenomenon”. Since 2010, transgenic peas were officially released into environment for multiplication and tests of agronomic performance. Preliminary feeding tests on rats did not show any negative effect of feed mixtures enriched with transgenic pea seed meal on the growth and health conditions of experimental animals. Further, construct with gmSPI-2 protease inhibitor was used for the development of fungus and insect resistance. Pea transgenic lines that generated increased mortality of Bruchus pisorum larvae were achieved. Acknowledgements The work is financially supported by National Agency for Agricultural Research of Ministry of Agriculture CR, grant No. QI91A229. 176
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First Legume Society Conference 201
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Scientific Committee Michael Abbert
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Programme 9 Session 1 Achievements
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