here - First Legume Society Conference (LSC1)

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Breeding research for abiotic stresses tolerance in faba bean (Vicia faba L.)
Mohamed Ali 1,2 , Gregor Welna 1 , Ahmed Sallam 1 , Christiane Balko 3 , Olaf Sass 4 , Mehmet
Senbayram 5 , Regina Martsch 1 , Wolfgang Link 1
1 Department of Crop Science, Göttingen University, Germany
2 Agronomy Department, Faculty of Agriculture, Assiut University, Egypt
3 Julius Kühn Institute, Groß Lüsewitz, Sanitz, Germany
4 Norddeutsche Pflanzenzucht, Hans-Georg Lembke KG, Germany
5 Institute of Applied Plant Nutrition, Göttingen University, Germany
Rapid powerful approaches for identifying quantitative trait loci (QTL) and DNA markers tightly
associated with drought and frost stress tolerance are prerequisites for marker-assisted selection
and breeding of faba bean (Vicia faba L.) for areas prone to such stresses. Our faba bean breeding
research focuses mainly on winter types. We use SSD lines (our so-called association set, A-set), a
verification-set (V-set; subset of A-set), a first mapping set (K-set), and a set of back-cross
families developed from some of the A-set lines. These aforementioned genetic materials are
being used to map drought and frost stress tolerance separately and jointly using AFLP, RAPD
and SNP-markers (altogether about 900 markers). The phenotyping of drought stress tolerance is
being conducted using rain-out-shelters and chemical desiccation in the field, applying drought
stress in pot trials (greenhouse) with juvenile plants, and determining physiological parameters in
leaf disc/single leaf tests. On the other hand, for frost stress tolerance, the phenotyping is
conducted in the field as well as in so-called frost chamber (juvenile plants in pots). Under frost
stress, loss of leaf turgidity (h 2 =0.84) was correlated with disposition to survive (r=-0.76 ** ).
Moreover, wilting score (h 2 =0.52) was correlated with dry matter content (r=0.55 ** ) under
drought stress. A main outcome of our project will be a list of useful, well-marked QTLs for
drought and frost stress tolerance in winter faba bean. Moreover, we are currently identifying
reliable screening tools to phenotype these traits and we are identifying highly promising parental
lines for applied breeding purposes.
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