here - First Legume Society Conference (LSC1)

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Comparative analysis of GA2ox genes encoding enzymes involved in gibberellin
deactivation in different species of lupines
Katarzyna Marciniak 1 , Marika Haręcka 1 , Emilia Wilmowicz 1 , Agata Kućko 1 , Paulina Glazińska 1 ,
Waldemar Wojciechowski 1 , Mariusz Banach 1 , Jacek Kęsy 1 , Jan Kopcewicz 1 , Andrzej Tretyn 2
1 Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Toruń, Poland
2 Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, Toruń, Poland
The crucial moment to achieve high productivity of legumes is correctly running process of
flower growth and development. In different species of lupines this stage is associated with
undesirable occurrence, which is the falling of flowers. In Arabidopsis thaliana or cereals is known
that important role in flowering play a gibberellins. Therefore, it was decided to identify the genes
which encode the enzymes responsible for the phytohormone deactivation in such lupine species
as Lupinus albus (butan, boros), Lupinus luteus (taper, mister) and Lupinus angustifolius (kadryl,
sonet). Using PCR method and degenerate primers six fragments of GA2ox genes (492-498 nt)
were amplified. These sequences were subjected to bioinformatic analysis and in this manner
obtained protein fragments (164-166 aa). The presence of characteristic domains determined
using BlastP and ExPASy programs indicates with a high probability that they are part of the
functional enzyme proteins. Further analysis showed a high degree of identity of nucleotide and
amino acid sequences (98-99%) between the varieties of the same species of lupine. In turn
interspecies comparison of lupines showed a significant degree of similarity between sequences
from L. albus and L. luteus (84%) and less in the case of L. angustifolius (54-56%). All known
sequences also exhibit a relatively high degree of similarity to other plant species, which indicates
their conservatism during the evolution. The presented results are just an introduction to the
extensive research that in the near future will determine the precise mechanism of flowering in
three species of lupines.
Acknowledgdements
This work is supported by Ministry of Agriculture and Rural Development of Poland Grant no 149/2011
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