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Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Antioxidant capacity of yellow and black soybean seed coat and dehulled bean in relation
to their distributions of isoflavones, anthocyanins and phenolic acids
Slañana Žilić 1a , Vural Gökmen 2b , Arda Serpen 2b , Vesna Perić 1b , Gül Akıllıoğlu 2
1 Maize Research Institute, a Department of Technology and b Breeding Department, Belgrade-Zemun, Serbia
2 Department of Food Engineering, b Food Research Center, Hacettepe University, Ankara, Turkey
Recently, attention has been given to the significant potential of soybean in the prevention of
chronic diseases such as atherosclerosis, coronary heart disease and cancer. Given that oxidative
damage represent one of the mechanisms leading to chronic diseases many researches have been
focused on the antioxidant compounds in soybeans. In this study, the total and individual
phenolic profiles and their contribution to antioxidant capacities of seed coats and dehulled beans
of black and yellow soybeans were investigated. Black seed coats had the highest content of total
phenols, flavonoids and anthocyanins, in addition the highest catechin and protocatechin
contents. On the other hand, dehulled beans from yellow soybeans had the highest levels of
ferulic and p-coumaric acid content, and free and bound isoflavone content. The present study
demonstrated that anthocyanins predominantly accounted for the difference in polyphenols
between black soybeans and other yellow soybean genotypes. According to our study, the average
value of black seed coat samples for the anthocyanins content was 13955.01 mg CGE/kg. Due to
the highest phenolic index, the black seed coats from soybean genotypes Black Tokio and
Cornaja had the highest ABTS •+ scavenging activity (597.46 and 486.15 mmol Trolox/kg,
respectively). The results suggest that the black seed coat, as well as dehulled bean from yellow
soybeans would potentially provide sources of natural antioxidants that may play a crucial role in
the prevention of oxidation-related diseases.
Acknoledgements
This study was financially supported by the Ministry of Education and Science of the Republic of Serbia (Grants no.
TR-31069).
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