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Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013 _________________________________________________________________________________________ Saturation of a Quantitative Trait Loci controlling resistance to Orobanche crenata in pea María José Cobos 1 , Diego Rubiales 1 , Sara Fondevilla 1,2 1 Institute for Sustainable Agriculture – CSIC, Córdoba, Spain 2 University of Frankfurt, Frankfurt am Main, Germany Crenate broomrape (Orobanche crenata) is a root parasitic weed that represents a major constraint for pea cultivation in the Mediterranean Basin and Middle East. In a previous study we identified several Quantitative Trait Loci (QTLs) associated with resistance to O. crenata in the pea RIL population P665 x Messire. A QTL located in LGVII was associated with a lower number of O. crenata tubercles per root length and explained a high proportion of the phenotypic variation of the trait (33%). In this study this genomic region was saturated with new markers by comparative mapping with Medicago truncatula. Nine molecular markers previously mapped in P. sativum LGVII and in M. truncatula were analysed in P665 x Messire. Eight of them could be included in the map, being dFPA2maker located into de QTL. In addition, a SCAR marker (SCARK6) was developed from a RAPD marker tightly linked to the QTL. The inclusion in our map of these markers and the M. truncatula EST DNABP allowed the comparison with M. truncatula. The pea region containing the QTL was located in M. truncatula chromosome 4. Thirty eight M. truncatula genes, expected to be located into the QTL region, were selected. Four of them could be mapped in LGVII in P665 x Messire genetic map. Two genes (Medtr4g068580.1 and Medtr4g068270.1) colocalized with the QTL. 197
Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013 _________________________________________________________________________________________ Studies on Water-Logging Tolerance in Pigeon pea: Future Prospectus Anupama Hingane, Suyash Patil, KB Saxena International Crops Research Institute for Semi-Arid Tropics, Patancheru, India Temporary water-logging during rainy season is a major yield reducer in pigeonpea. Since agronomic options are expensive some permanent genetic solutions are necessary to overcome this constraint. To identify tolerant cultivars, 49 genotypes were screened at three critical stages of crop growth. These genotypes were selected on the basis of initial screening in previous year. Screening at seed stage revealed that dark seed genotypes, long duration lines and hybrids exhibited greater tolerance to eight days of continuous water logging as compared to white or brown seeded lines. At early seedling stage, water-logging resulted in chlorosis, wilting of plants, reduction in leaf area and height in susceptible lines. While screening at late seedling stage in field conditions showed drastic effect of submergence on plant survival, there was severe phytophthora infestation in field screening and very few genotypes survive submergence at this stage. Genotypes ICP 5028, ICPH 2740, MAL 15, ICPH 2431, ICPL 20237, ICPL 87119 and ICPL 332 survived water-logging stress at all the three stages. Some anatomical and morphological traits were found to be related to Water-logging resistance. These include development of lenticles and adventitious roots just above the water level along with formation of arenchyma cells in root cortex. Linking tolerance with these adaptation traits may help in selecting resistance genotypes. Crosses have been made to study the inheritance of resistance and to develop populations for identifying molecular markers. This will enable in pyramiding the desirable traits to improve submergence tolerance through marker assisted selection. 198
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First Legume Society Conference 201
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Scientific Committee Michael Abbert
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Programme 9 Session 1 Achievements
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