here - First Legume Society Conference (LSC1)

Recommendations

Info

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013 _________________________________________________________________________________________ QTL detection for partial resistance against Didymella pinodes in two connected mapping populations of the model plant Medicago truncatula Eva Madrid 1 , Eleonora Barilli 1 , Teresa Millán 2 , Thierry Huguet 3 , Laurent Gentzbittel 4 , Diego Rubiales 1 1 Institute for Sustainable Agriculture-CSIC, Córdoba, Spain 2 University of Córdoba, Department of Genetics, Córdoba, Spain 3 University of Toulouse, EcoLab, Castanet Tolosan, France 4 INP-Agro Toulouse, France Ascochyta blight incited by Didymella pinodes (formerly Mycosphaerella pinodes) is one of the most important fungal diseases of pea worldwide. In this study searched for Quantitative Trait Loci (QTL) regions associated with quantitative resistance to D. pinodes in the model legume Medicago truncatula. The analysis was carried out using two connected mapping populations at F 8 derived from two crosses (J6 x F83005.5 and J6 x DZA315.16), being J6 moderately susceptible and both F83005.5 and DZA315.16 resistant to D. pinodes infection. Parental lines and RILs population were macroscopically evaluated in terms of relative Disease Severity (rDS). In order to provide more effective detection and evaluation of the effects of the QTLs and their stability and fine mapping of candidate genes annotated on the M. truncatula sequence, a combined genetic map using both population was developed. The QTL analysis revealed a QTL in the LG2 explaining up to 13% of the total phenotypic variation for rDS to D. pinodes. Two SSR markers, MTE80 and mtic890 (3 cM apart) were the most significantly associated. The markers are located in BAC AC119409 and BAC AC125474, respectively. These two BACs are overlapping on M. truncatula chromosome 2. The integration of QTL analysis and genomics in M. truncatula will facilitate the identification of candidate genes related with host resistance. 161
Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013 _________________________________________________________________________________________ The role of signal molecules in defence response of pea (Pisum sativum L.) to pea aphid (Acyrthosiphon pisum Harris) infestation Van Chung Mai 1,2 , Iwona Morkunas 1 1 Department of Plant Physiology, Poznań University of Life Sciences, Poznań, Poland 2 Department of Plant Physiology, Faculty of Biology, University of Vinh, Vinh, Vietnam Pea aphid (Acyrthosiphon pisum Harris) is a destructive pest of leguminous plants worldwide. Damages of pea seedlings leaves (Pisum sativum L.cv. Cysterski) by pea aphid A. pisum were dependent on the intensity and duration of infestation. It has been revealed that in pea seedlings leaves in responses to A. pisum infestation were induced accumulation of phytohormones that is salicylic acid (SA), jasmonic acid (JA), ethylene (ET), and production of reactive oxygen/nitrogen species (ROS/RNS) such as hydrogen peroxide (H2O2) and nitric oxide (NO). Following aphid infestation, these signal molecules were induced at different points of time. Strong generation of H2O2 and JA were observed in pea seedlings leaves at 24 hpi. In turn, after the induction of ET and NO at 48 hpi in aphid-infested leaves the generation of SA occurred (strongly increased from 72 hpi to 96 hpi). Then, JA and ET were again induced and reached the second maximum levels at 96 hpi. Therefore, at the point of 96 hpi there was the combined action of three phytohormones SA, JA and ET in the defense mechanism of pea leaves. In parallel, the increase in activity of enzymes phenylalanine ammonia-lyase (PAL) and benzoic acid 2-hydroxylase (BA2H) in the SA synthesis pathway, lipoxygenase (LOX) engaged with the biosynthesis of JA was similar to the enhanced alteration of these signal molecules. The antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) are important elements of the regulation of H2O2 metabolism in responses to oxidative stress. The increase in activity the antioxidant enzymes were probably connected with the defensive role of H2O2 and reduction of oxidative ·- stress in pea leaves. Furthermore, the accumulation of superoxide anion (O2 ) and semiquinone radicals was involved in the defense responses of pea leaves to pea aphid. In pea cells, the ·- increase generation of O2 played a defensive role to protect against aphid herbivory, whereas semiquinones may contribute to build-up of a barrier or activate other defense strategies. The level of these radicals increased progressively in response to the attack of pea aphids. These aspects contribute to the novel knowledge concerning the identification of regulatory mechanisms during the plant-aphid interactions. Acknowledgements The study is supported by the Polish National Science Centre Project (NCN, grant no. 2011/01/B/NZ9/00074) 162
Page 2:
First Legume Society Conference 201
Page 5 and 6:
Scientific Committee Michael Abbert
Page 8:
Programme 9 Session 1 Achievements
Page 12 and 13:
Book of Abstracts First Legume Soci
Page 14:
Page 18 and 19:
Page 20 and 21:
Page 22 and 23:
Page 24 and 25:
Page 26 and 27:
Page 28 and 29:
Page 30 and 31:
Page 32 and 33:
Page 34 and 35:
Page 36 and 37:
Page 38 and 39:
Page 40 and 41:
Page 42 and 43:
Page 44 and 45:
Page 46:
Page 50 and 51:
Page 52 and 53:
Page 54 and 55:
Page 56 and 57:
Page 58 and 59:
Page 60 and 61:
Page 62 and 63:
Page 64 and 65:
Page 66 and 67:
Page 68:
Page 72 and 73:
Page 74 and 75:
Page 76 and 77:
Page 78 and 79:
Page 80 and 81:
Page 82 and 83:
Page 84 and 85:
Page 86 and 87:
Page 88 and 89:
Page 90 and 91:
Page 92 and 93:
Page 94 and 95:
Page 96 and 97:
Page 98 and 99:
Page 100 and 101:
Page 102 and 103:
Page 104 and 105:
Page 106 and 107:
Page 108 and 109:
Page 110 and 111:
Page 112 and 113: Book of Abstracts First Legume Soci
Page 114: Book of Abstracts First Legume Soci
Page 138: Session 6 Translational omics for l
Page 161: Book of Abstracts First Legume Soci
Page 212 and 213:
Page 214 and 215:
Page 216 and 217:
Page 218 and 219:
Page 220 and 221:
Page 222 and 223:
Page 224 and 225:
Page 226:
Session 8 Non-food, non-feed and ot
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
Page 235 and 236:
Page 238 and 239:
Page 240 and 241:
Page 242 and 243:
Page 244 and 245:
Page 246 and 247:
Page 248 and 249:
Page 250 and 251:
Page 252 and 253:
Page 254 and 255:
Page 256 and 257:
Page 258 and 259:
Page 260 and 261:
Page 262 and 263:
Page 264 and 265:
Page 266 and 267:
Page 268 and 269:
Page 270 and 271:
Page 272 and 273:
Page 274:
Page 278 and 279:
Page 280 and 281:
Page 282 and 283:
Page 284 and 285:
Page 286 and 287:
Page 288:
Page 292 and 293:
Page 294 and 295:
Page 296 and 297:
Page 298 and 299:
Page 300 and 301:
Page 302 and 303:
Page 304:
Page 308 and 309:
Page 310 and 311:
Page 312:
Page 315 and 316:
Name Page Name Page Capel J 182 Duc
Page 317 and 318:
Name Page Name Page Klenotičová H
Page 319 and 320:
Name Page Name Page Prusiński J 25
Page 322 and 323:
What is IFVCNS? PLATINUM SPONSOR In
Page 324 and 325:
Agromarket www.agromarket.rs Agroma
Page 326 and 327:
CID Bio-Science, Inc. www.cid-inc.c
Page 328 and 329:
Saatzucht Steinach GmbH & Co KG Don
show all

here - First Legume Society Conference (LSC1)

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?