here - First Legume Society Conference (LSC1)

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Analysis of eIF4E gene sconfering resistance to Pea seed-borne mosaic virus (PSbMV)
and other potyviruses in pea
Petr Smýkal 1 , Eva Konečná 2 , Pavel Hanáček 2 , Gregoire Aubert 3 , Dana Šafářová 4 , Milan Navrátil 4
1
Department of Botany, Faculty of Science, Palacký University, Olomouc, Czech Republic
2
Mendel University, Department of Plant Biology, Faculty of Agronomy Brno, Czech Republic
3
Unité Mixte de Recherches en Génétique et Ecophysiologie des Légumineuses à Graines, INRA-UMRLEG,
Dijon, France
4
Department of Cell Biology and Genetics, Faculty of Science, Palacký University,Olomouc, Czech Republic
Pea seed-borne mosaic virus (PSbMV) belongs among the most frequent viral pathogens causing
severe losses in field pea and other legumes. These losses might be prevented by growth of
resistant varieties. Resistance to the common P1 strain of PSbMV isconferred by a single
recessive gene (eIF4E), localized on LG VI (sbm-1 locus), while to lentil L1 strainis localized on
LGII atsbm-2locus. Gene-specific single nucleotide polymorphism and co-dominant amplicon
length polymorphism markers were developed. From the comparison of genotype verses
phenotype for selection of resistance/susceptibility in F 2 plants, there was a 26% discrepancy
between the PCR and ELISA-based assays with potentially susceptible heterozygote plants
missed. We used qRT-PCR to analyze expression of both resistant and sensitive eIF4E alleles in
heterozygotes, sensitive and resistant homozygotes after PSbMV infection, together with
evaluation of viral concentration. The novel eIF4E alleles were detected in wider pea germplasm
and TILLING mutants and these were virologically tested. We have analyzed eIF4E allelic
variation in over 2000 accesions within geographical context. Furthemore, analysis of second sbm-
2 locus with eIF4E iso and eIF4G genes as candidate targets, will be presented. It can be expected
that information gain in this study might be extended to other legume species, particularly to
economically important species of tribus Fabeae or even to subfamily Papilionoideae such as
chickpea, lentil, faba bean or soybean where Potyviruses cause problems.
Acknowledgements:
The work was supported by NAZV QI91A229 project.
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