here - First Legume Society Conference (LSC1)

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Genetic diversity assessment in soybean cultivars using morphological and molecular
marker data
Ana Nikolić, Vesna Perić, Mirjana Srebrić, Snežana Mladenović Drinić
Maize Research Institute „Zemun Polje“, Belgrade, Serbia
Eighteen soybean genotypes from three different breeding institutions were evaluated using
morphological and molecular marker data. Thirteen qualitative traits were chosen for
morphological characterization. RAPD molecular marker analysis was used to deterimne genetic
diversity and relatedness between soybean cultivars. Diversity of qualitative traits was described
by Shannon index and its average value was 0.766. Polymorphism information content (PIC) was
calculated for RAPD data with average value of 0.389. Genetic similarity was determined using
simple matching coefficient for all types of data and it ranged from 0.278 to 0.888 for phenotypic
and from 0.740 to 0.972 for molecular markers. Genetic similarity matrices were submitted to
cluster analysis using UPGMA method in NTSYS-pc software. Relative positions of the soybean
cultivars in dendrograms based on qualitative and RAPD data were rather different. Results of
cluster analysis based on molecular data were in better agreement with pedigree data. Both
systems (molecular and morphological) together provide different and complementary
information. Genetic relationships estimated with both molecular markers and phenotypic traits
can reveal sources of desirable characteristics in closely related individuals.
Acknowledgements
The project TR-31068 of the Ministry of Education, Science and Technological Development of the Republic of
Serbia
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