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Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013 _________________________________________________________________________________________ Improving the efficiency of biological nitrogen fixation of chickpea Cicer arietinum L. Mariia Donskaia 1 , Andrej Vasilchikov 1 , Tatiana Naumkina 1 , Galina Suvorova 1 , Vladimir Naumkin 2 1 The All-Russia Research Institute of Legumes and Groat Crops, Orel, Russia 2 Orel State Agrarian University, Orel, Russia Chickpea Cicer arietinum L. is characterized by high dietary diversity as a food crop. At the same time it has a high resistance to drought and heat. In the Russian Federation the chickpea is grown on the area over 100 thousand hectares. It can be used as an insurance culture instead of peas in the central regions of Russia with frequent droughts. Introduction of chickpea in the north regions is restrained mainly because of the lack of well-adapted varieties with short growth period and crop management practice. Inoculation with nitrogen fixing bacteria Mesorhizobium ciceri (strain 527 ARRIAM) and fungi of arbuscular mycorrhiza Glomus was applied in order to increase nitrogen fixation efficiency. Research was carried out in 2010-2012 at the field of the All-Russia Research Institute of Legumes and Groat Crops on the dark-gray forest soils with a humus content of 4,7 - 4,9%. 13 chickpea accessions received from the Vavilov Research Institute were tested in this study. Use of microbiological agents increased plant height by 0,9 – 9,4%. Maximum increase of seed yield per plant of 61,1- 69,4% was observed in variant with dual inoculation with rhizobia and fungi. Maximum amount of nodules formed in case of single rhizobia inoculation and was 79 nodules per plant, maximum nitrogenase activity was recorded at the level of 256 µg N 2/plant/h in variant with dual inoculation. Total seed yield of chickpea in variants with microbe inoculation increased by 0,2 – 0,7 t/ha over control. Acknowledgements The reported study was supported by RFBR, research project No.12-04-97552 281
Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013 _________________________________________________________________________________________ Phenotypic and genetic diversity of rhizobia isolated from nodules of the legume genera Hedysarum coronarium in North West region of Morocco Fatima Ezzakkioui 1 , Nourdin El Mourabit 2 , Said Barrijal 1 1 Laboratoire de Valorisation Biotechnologique des Microorganismes Génomique et Bioinformatique, FSTT 2 CRRA,INRA, Tanger The species Hedysarum coronarium L. (tribe Hedysareae, family Leguminosae), known by the Italian name of sulla or Spanish sainfoin, ranges within the Mediterranean basin from northern Africa to southern Spain and in Italy from centrally to southern. In Morocco these phytogenetic resources are currently damaged by severe genetic erosion due to overgrazing, irregular rainfall and reduction of rangeland. Phenotypic and genetic diversity of 60 strains, isolated from root nodules of Hedysarum coronarium in North West region of Morocco, were studied using both phenotypic and genetic techniques. The phenotypic tests, (growth rate, tolerance to salinity, pH, temperature, resistance to antibiotics and metals ...), revealed that most of strains are belonging to rhizobia and genetic tests clustered all strains into 26 different profiles. Results of 16S rRNA sequencing revealed that Hedysarum rhizobia were phylogenetically related to Rhizobium sullae strain IS 123T in 14 groups. In this study, we revealed that a large diversity among the 60 strains. 282
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First Legume Society Conference 201
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Scientific Committee Michael Abbert
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Programme 9 Session 1 Achievements
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Session 8 Non-food, non-feed and ot
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