here - First Legume Society Conference (LSC1)

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Identification of genes involved in resistance to Didymella pinodes in pea by
deepSuperSAGE genome-wide transcriptome profiling
Sara Fondevilla 1,2 , Björn Rotter 3 , Nicolas Krezdorn 3 , Ruth Jüngling 3 , Peter Winter 3 , Diego
Rubiales 1
1 Institute for Sustainable Agriculture, CSIC, Córdoba, Spain
2 University of Frankfurt, Biocenter, Frankfurt, Germany
3 GenXPro GmbH, Frankfurt am Main, Germany
Didymella pinodes, causing ascochyta blight, is one of the most important pea pathogens. Despite
the devastating consequences of this disease, very little is known about the mechanisms of
resistance in the host. We employed the open-architecture transcriptome profiling technique
deepSuperSAGE, coupled with next-generation sequencing, to identify pea-specific genes
involved in the resistance to this important disease in the resistant Pisum sativum ssp. syriacum
accession P665.Two deepSuperSAGE libraries were constructed from leaf RNA of infected and
control plants, yielding a total of 17,561 different UniTags. 70% of them could be assigned to
known sequences from pea or other plants. 509 UniTags were significantly differentially
expressed (p < 0.05; fold change ≥ 2, ≤ 2) in inoculated versus control plants. This study
provides a detailed picture of all expressed genes and metabolic pathways differentially regulated
during D. pinodes-Pisum sativum interaction and contribute to the identification of candidate
resistance genes. Protease inhibitors, antifungal compounds, strengthening of host cell walls,
detoxification of D. pinodes toxins and repair of cell walls could contribute to resistance. Ethylene,
ABA and indole-3-acetic acid pathways were up-, while the GA pathway was down-regulated.
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