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Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013 _________________________________________________________________________________________ Phytase have beneficial effect in lupine diets for broiler chickens Sebastian Kaczmarek, Marcin Hejdysz, Andrzej Rutkowski Poznan University of Life Science, Poznan, Poland The aim of the study was to investigate the effect of lupine (yellow) in three different levels (5, 10, 20 %) and phytase addition on broiler chickens performance, Ca and P apparent retention as well as tibia ash. The experiment was conducted with 512 broiler male chickens, divided into eight dietary treatments, 8 replications with 8 birds in each. All of diets were deficient in Ca, available P and lysine and TSAA. Apparent ileal digestibility (AID) and retention (AIR) was determined using TiO 2 as a digestibility marker. Statistical analysis of results was performed using the General Linear Models procedure (GLM) of the SAS ® . Use of phytase improved BWG (1747 vs. 1872 g), (p
Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013 _________________________________________________________________________________________ Antioxidant characterization of phenolics from Trifolium scabrum L. aerial parts Iwona Kowalska 1 , Łukasz Cieśla 2 , Anna Stochmal 1 1 Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation-State Research Institute, Pulawy, Poland 2 Department of Inorganic Chemistry, Medical University, Lublin, Poland Phenolic compounds have been recognized for many years as potent antioxidants. Isoflavones are naturally occurring diphenolics found mainly in the legume family. Our preliminary analysis suggest that Trifolium scabrum L. (rough clover) can be considered as very good source of these compounds for commercial use. Fourteen isoflavones (aglycones or their glucosylated derivatives) and two flavonol glucosides have been isolated and identified in aerial parts of Trifolium scabrum L. Free radical scavenging potential of these substances has been assessed by means of a simple benchtop TLC-DPPH • bioassay. Standard compounds and the analyzed fraction were applied to aluminium-backed silica gel chromatographic plates. As the analyzed extract contained compounds characterized with a wide range of polarity different chromatographic systems were used to screen the free radical scavenging potential of individual compounds. In this study following mobile phases were used: toluene-ethyl acetate-formic acid (60:30:5, v/v/v) – for the least polar compounds; ethyl acetate-formic acid (95:5, v/v) – for the medium polar constituents and chloroform-methanol-ethyl acetate-water (20:40:22:10, v/v/v/v) for the most polar ones. TLC plates were immersed in freshly prepared 0.2% (w/v) methanolic DPPH • solution. After removing DPPH • excess, plates were kept in the dark for 30 min and then scanned by flatbed scanner. A comparison of the results was performed by an image processing program ImageJ. Antiradical activity was shown by following compounds: 3’-hydroxydaidzein-7- O-glc, isoquercitrin, calycosin-7-O-glc, kaempferol-3-O-glc (astragalin), daidzein, genistein and biochanin A. Acknowledgements Participation in the First Legume Society Conference (LSC1) was sponsored by European Community 7 th Framework Program project “Proficiency” (FP7REGPOT-2009-1-245751). 298
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First Legume Society Conference 201
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Scientific Committee Michael Abbert
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Programme 9 Session 1 Achievements
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Session 6 Translational omics for l
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Session 8 Non-food, non-feed and ot
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