here - First Legume Society Conference (LSC1)

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Use of molecular markers for detection of ascochyta blight and fusarium wilt resistance
in Tunisian chickpea breeding lines
Mariem Bouhadida 1 , Rim Ben Jannet 1 , Warda Jendoubi 1 , Moez Amri 2 , Mohamed Kharrat 1,3
1
University of Carthage, Field Crop Laboratory, National Institute for Agricultural Research of Tunisia,
Tunisia
2
Field Crop Laboratory, Regional Field Crop Research Center of Béja, Béja, Tunisia
3
Intenational Center for Agricultural Research in the Dry Areas, Rabat-Instituts, Rabat, Morocco
Twenty three chickpea cultivars, mainly advanced lines and some Tunisian varieties released by
the national chickpea breeding program, have been studied for their resistance to the two most
damaging diseases: ascochyta blight (AB) and fusarium wilt (FW). The field evaluation for AB
was conducted in two field locations and under controlled conditions. Two co-dominant markers
associated with AB resistance were also used in this study; the CaETR marker tightly linked to
QTL AR1 in combination with the SCAR SCY17 590 marker linked to another QTL AR2. These two
markers contribute efficiently in the selection of new chickpea varieties with the better
combination of alleles to ensure a durable resistance for AB and are very useful in the chickpea
breeding program. The same genotypes were also subject to FW field evaluation during two
cropping season and under controlled conditions. Four STMS markers associated with FW
resistance were also used, three of them (TA27, TA59 and TA96) were found tightly linked to the
second gene controlling the resistance to race 0 of FW. While the fourth STMS used in this study
(TR59) was closely linked to the first gene of resistance. The dendrogram obtained from the
molecular profiles with these 4 STMS markers does not classify the studied genotypes according
to their behavior toward FW. In fact, these markers are described to have extensive
polymorphism. Thus, resistance allele prediction with STMS is difficult. The development of new
stable marker as diagnostic or SCAR markers is on-going for genotyping correctly susceptible and
resistant chickpea lines to FW which could be very interesting in assisting chickpea breeding
program.
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