here - First Legume Society Conference (LSC1)

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Yield relationships between F 2 and F 3 soybean lines differing in homozygosity for the
presence of the Kunitz trypsin inhibitor
Mirjana Srebrić, Vesna Perić, D Kovačević, Vesna Dragičević, Snežana Mladenović Drinić
Maize Research Institute Zemun Polje, Belgrade, Serbia
An F 2 population from the cross combination between Kunitz-free soybean variety Laura and
common grain type variety Afrodita was developed. Satt228 marker, tightly linked to Kunitz
trypsin inhibitor (KTI) locus, was used to screen 92 individual F 2 plants in order to identify
homozygous and heterozygous genotypes for KTI alleles. The genotypic ratio was 1:2:1 (Ti TI :
Ti ti : ti ti constitution) which is expected for a character determined by a single mendelian gene.
The seed yield of individual F 2 plants, as well as yield of their F 3 progenies, was measured. In
order to determine yield relationships between F 2 and F 3 soybean lines, simple correlations and
Spearman’s rank correlation was calculated. Dependences between yield of F 2 and F 3 soybean
lines was not found into whole three genotypic classes. Because of that early generation selection
should not to be rigorous. An SSR marker Satt228 can be useful tool for early Identification of
homozygous and heterozygous genotypes in breeding for Kunitz-free soybean varieties, with no
potential risk for loosing high yielding genotypes. Presence or absence of KTI did not influence
yield relationships between two early generations (F 2 and F 3) of soybean.
Acknowledgements
This research is a part of the project TR-31068 of the Ministry of Education and Science of the Republic of Serbia
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