here - First Legume Society Conference (LSC1)

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Molecular cloning of complete cDNA and examination the expression level of
homologue miR167 target gene LlARF6 in Lupinus luteus
Paulina Glazińska 1,2 , Waldemar Wojciechowski 1,2 , Emilia Wilmowicz 1,2 , Katarzyna Marciniak 1,2 ,
Jacek Kęsy 1,2 , Jan Kopcewicz 1
1
Chair of Plant Physiology and Biotechnology, Faculty of Biology and Environment Protection, Nicolaus
Copernicus University, Poland
2
Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, Poland
The plant hormone auxin regulates a broad spectrum of developmental processes. Recent
advances have been made in the understanding of auxin response pathways, primarily by the
characterization of genes encoding Auxin Response Factors (ARF) in Arabidopsis. In addition,
expression of some of ARFs is regulated by microRNAs (miRNAs). In A. thaliana, ARF6 and
ARF8 are targeted by miR167 whereas ARF10, ARF16, ARF17 are targeted by miR160.
Nevertheless, little is known about possible interactions between miRNAs and auxin signaling
pathway during plant development in another plant species. In this study, we identify and
examine the expression level of homologue miR167 target gene LlARF6 in Lupinus luteus. The
identified cDNA contains miR167 complementary sequence and shows significant similarity to
ARF6 cDNAs of other plant species. Predicted amino acid sequence of LlARF6 includes all
characteristic domains for ARF family transcription factors (B3 DNA-binding domain,
AUX/IAA - CTD and a glutamine-rich region). Accumulation of LlARF6 mRNA was examined
in the vegetative and generative organs L.luteus. Our results may suggest potential role of LlARF6
in regulation sach proceses as its homologues in other plant species.
Acknowledgements
The work was supported by the Multi-Year Programme of the Polish Ministry of Agriculture and Rural Development,
No. 149/2011.
HTCE “Park” a.d. Novi Sad, Novosadskog Sajma 35, Serbia
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