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Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Physiological, biochemical and molecular variations for cold tolerance in desi and kabuli
chickpea (Cicer arietinum L.) genotypes
Jagmeet Kaur 1 , Ritika Aggarwal 2 , Satvir Kaur 3
1 Department of Plant Breeding and Genetics, PAU, Ludhiana
2 Department of Botany, PAU, Ludhiana
3 Department of Biochemistry, PAU, Ludhiana
The present investigation was carried out to assess the variations in physiological, biochemical
and molecular traits in desi (PBG1, PBG 5, GL28008 and GNG1861) and kabuli (L552, GLK
26162) chickpea cultivars contributing towards cold stress tolerance in them at various growth
stages and at temperature maintained 5 0 C and 10 0 C during crop development. The higher
biomass accumulation in kabuli chickpea cultivars at low temperature was due to profuse
vegetative growth in them as indicated by increased plant height, leaf area and leaf area index in
them. Physiological parameters such as leaf water content and respiratory activity was found to
be higher in kabuli genotypes at all the growth stages whereas photosynthetic efficiency in terms
of total chlorophyll content and photosynthetic assimilation rate was recorded to be higher in
desi genotypes. Pollen viability was found to be maximum in L552 (81.8%) followed by
GLK26162 (76.9%) and GNG1861 (73.8%). The higher status of antioxidative enzymes (catalase
and peroxidase) along with proline content in GNG1861 and GLK 26162 at lower temperature
might be responsible for their tolerance behaviour towards cold stress tolerance. The highest
yield was recorded in GNG1861 (3185 Kg/ha) followed by L552 (3055 Kg/ha). GLK 26162
showed distinct physiological and biochemical characters which render it a tolerant in kabuli
category with 81.3% filled pods and highest 100 seed weight of 31.6 g. All these cultivars were
characterized for the molecular diversity using nineteen RAPD primers. A dendrogram
constructed on the basis of UPGMA clustering method revealed grouping of these genotypes in
two major clusters except for kabuli genotype GLK 26162. Cluster-I constituted four genotypes
grouped into two sub clusters- A and B. Highly tolerant desi genotype GNG1861 with 76.4%
filled pods and moderately tolerant kabuli genotype L552 with 82.5% filled pods were found to
be closely related in sub cluster B having maximum Jaccard’s similarity coefficient. The most
sensitive genotype PBG 5 having minimum filled pods (65.2%) was found alone in the cluster –
II.
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