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Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Development of large seeded chickpea lines resistant to Ascochyta blight
Josefa Rubio 1 , Eva Madrid 2 , Juan Gil 3 , Teresa Millan 3
1 Área de Mejora y Biotecnología, IFAPA Centro “Alameda del Obispo”, Córdoba, Spain
2 Institute for Sustainable Agriculture, CSIC, Córdoba, Spain
3 Dpto Genética, Univ Córdoba, Campus de Excelencia Internacional, Campus de Rabanales, Córdoba, Spain
With the aim of developing chickpea cultivars including blight resistance and large seeds a
crossing program between 5 resistant advanced lines with medium-large seeds and different
sources of resistance were initiated in year 2005. Those advanced lines were originally derived
from crosses between resistant accessions from ICARDA and the Spanish large seeded (around
0.50 g per seed) and susceptible local cultivar ‘Blanco Lechoso’. A F 2 population with a total of
909 plants was obtained joining F 1 derived from all possible crosses between the 5 advanced
lines. Early generation testing method of F 2-derived lines was employed for large seed selection
until F 2:5 families. The best individuals from the best F 2:5 families were selected and advanced
selecting for large seeds until F 5:8. Finally, in 2011/2012 agronomic season, 30 F 5:8 advances lines
were sown following a randomized block design with two repetitions and parents as controls.
Eleven lines with larger seed size than the best parent (0.44 g per seed) were selected (ranking
from 0.49 g to 0.57 g per seed). These results suggest that transgressive segregation is present in
chickpea for seed size. In order to test the presence of QTLs for ascochyta blight in the selected
advance lines, allele-specific markers for QTL AR1 (CaETR) and QTL AR2 (SCY17 590) were
employed. Both QTLs were present in all selected lines.
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