Participación del Factor Silenciador Neuronal Restrictivo - Tesis ...

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121 Driever W, Solnica-Krezel L, Abdelilah S, Meyer D, Stemple D. (1997). Genetic analysis of pattern formation in the zebrafish neural plate. Cold. Spring. Harb. Symp. Quant. Biol. 62: 523-534. Dubois L, Bally-Cuif L, Crozatier M, Moreau J, Paquereau L, Vincent A. (1989). XCoe2, a transcription factor of the Col/Olf-1/EBF family involved in the specification of primary neurons in Xenopus. Curr. Biol. 8: 199-209. Durston AJ, Timmermans JPM, Hage WJ, Hendriks HFJ, Devries NJ, Heideveld M & Nieuwkoop PD. (1989). Retinoic acid causes an anteroposterior transformation in the developing central nervous system. Nature 340: 140-144. Eagleson G, Ferreiro B & Harris W. (1995). The fate of the anterior neural ridge and the morphogenesis of the Xenopus forebrain. J. Neurobiol. 28: 146-158. Ekker SC, McGrew LL, Lai CJ, Lee JJ, Von Kessler DP, Moon RY & Beachy PA. (1995). Distinct expression and shared activities of members of the hedgehog family of Xenopus laevis. Development 121: 2337-2347. Ermakova GV, Alexandrova EM, Kazanskaya OV, Vasiliev OL, Smith MW & Zaraisky AG .(1999). The homeobox gene, Xanf-1, can control both neural differentiation and patterning in the presumptive anterior neurectoderm of the Xenopuslaevis embryo. Development 126: 4513-4523. Fainsod A, Steinbeisser H, De Robertis EM. (1994). On the function of BMP-4 in patterning the marginal zone of the Xenopus embryo. EMBO J. 13: 5015–25. Feldman B, Gates MA, Egan ES, Dougan ST, Rennebeck G, Sirotkin HI, Schier AF, Talbot WS. (1998). Zebrafish organizer development and germ-layer formation require nodal-related signals. Nature 395: 181-185. Franco PG, Paganelli AR, Lopez SL & Carrasco AE. (1999). Functional association of retinoic acid and hedgehog signalling in Xenopus primary neurogenesis. Development 126: 4257–4265. Fuks F, Hurd PJ, Deplus R, Kouzarides T. (2003). The DNA methyltransferases associate with HP1 and the SUV39H1 histone methyltransferase. Nucleic. Acids. Res. 31: 2305-2312. Garcia-Bellido A. (1979). Genetic analysis of the achaete-scute system of Drosophila melanogaster. Genetics 91: 491-520. García-Castro MI, Marcell C & Bronner-Fraser M. (2002). Ectodermal Wnt function as a neural crest inducer. Science 297: 848 -851. Gershon A, Rudnick J, Kalam L, and Zimmerman K. (2000). The homeodomaincontaining gene Xdbx inhibits neuronal differentiation in the developing embryo. Development 127: 2945-2954.
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Page 1 and 2:
Universidad de Chile, Facultad de M
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Quisiera agradecer muy especialment
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Hipótesis……………………
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6. Función de XCoREST en el la esp
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Índice de figuras Figura 1. Experi
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Lista de publicaciones - Olguín P,
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embriones de Xenopus desde la blás
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laevis the ectoderm is patterned at
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Posteriormente, en otros vertebrado
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1.3 Inductores neurales y vías de
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formación de la placa neural y la
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Figura 3. Modelos de inducción neu
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destinos dependiendo de su localiza
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16 Figura 4. Modelo de doble gradie
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3.2 Neurogénesis primaria en Xenop
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adoptar el destino neural para fina
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neuronas ectópicas cuando es sobre
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Skarmeta et al., 1996) . Zic2 supri
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etrasan la diferenciación neuronal
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genes reporteros (ej. lacZ) que con
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manifiestan en la desacetilación d
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(Ballas et al., 2005) (Figura 11).
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corticales aislados y tratados con
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4.2.4 Función de REST/NRSF en neur
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1/NRSE mutado se expresa en gran pa
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silvestres. Sin embargo, en estadio
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tejido neural, como también en la
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participar en la neurogénesis a tr
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-Pinzas nº 5 (A. DUMONT y FILS, Su
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-MEMPFA (MOPS 0,1 M pH 7,0 EGTA 2 m
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-Bromuro de Etidio (GIBCO BRL, Cali
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concentración de mRNA a inyectar s
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Recolección de embriones. Para el
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Técnicas de Biología Molecular. A
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histona H3 fosforilada (Upstate) en
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Síntesis de ribosondas para hibrid
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Hibridación in situ (ISH). Los emb
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RESULTADOS 1. Clonamiento del cDNA
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Figura 14. Alineamiento de la secue
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3. Participación de XREST/NRSF en
Page 81 and 82: Figura 16. La interferencia de la f
Page 83 and 84: 3.2 La interferencia de la función
Page 85 and 86: 74 Figura 17. La interferencia de l
Page 87 and 88: 4. Participación de XREST/NRSF en
Page 89 and 90: Figura 19. La interferencia de la f
Page 91 and 92: 4.2 La sobreexpresión de XREST/NRS
Page 93 and 94: 4.3 Los fenotipos ectodérmicos aso
Page 95 and 96: 4.4 La sobreexpresión de XREST1 no
Page 97 and 98: 4.5 La interferencia de la función
Page 99 and 100: 4.6 XREST/NRSF participa en la form
Page 101 and 102: Figura 24. Los fenotipos asociados
Page 103 and 104: Para poner a prueba esta idea se co
Page 105 and 106: 6. XCoREST participa en el establec
Page 107 and 108: Figura 26. XCoREST participa en el
Page 109 and 110: 1. XREST/NRSF regula la expresión
Page 111 and 112: 100 2. La interferencia de la funci
Page 113 and 114: 102 actividad de Wnt y FGF inducen
Page 115 and 116: 104 letalidad, probablemente porque
Page 117 and 118: 106 XREST/NRSF neuraliza los explan
Page 119 and 120: 108 mutada en los dominios de fosfo
Page 121 and 122: 110 5. XREST/NRSF modula la adquisi
Page 123 and 124: 112 cuenta de un potencial de acci
Page 125 and 126: 114 al neuroectodermo. La inhibici
Page 127 and 128: 116 embargo, estos resultados deben
Page 129 and 130: 118 Ballas N, Battaglioli E, Atouf
Page 131: 120 Chitnis AB. (1999). Control of
Page 135 and 136: 124 Kallunki P, Edelman GM & Jones
Page 137 and 138: 126 Luther WH. (1935). Entwicklungs
Page 139 and 140: 128 Oppenheimer JM. (1936b). Transp
Page 141 and 142: 130 Spemann H & Mangold H. (1924).
Page 143 and 144: 132 Wilson PA, Hemmati-Brivanlou A.
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Participación del Factor Silenciador Neuronal Restrictivo - Tesis ...

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