Participación del Factor Silenciador Neuronal Restrictivo - Tesis ...

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125 LaBonne C & Bronner-Fraser M. (1998). Neural crest induction in Xenopus, evidence for a two-signal model. Development 125: 2403-2414. LaBonne C and Whitman M. (1997). Localization of MAP kinase activity in early Xenopus embryos: implications for endogenous FGF signaling. Dev. Biol. 183: 9-20. Lall S, Patel NH. (2001). Conservation and divergence in molecular mechanisms of axis formation. Annu. Rev. Genet. 35:407–37. Lamar E, Kintner C, Goulding M, (2001). Identification of NKL, a novel Gli–Kruppel zinc-finger protein that promotes neuronal differentiation. Development 128: 1335– 1346. Lamb TM, Knecht AK, Smith WC, Stachel SE, Economides A., Stahl N, Yancopolous GD & Harland RM. (1993). Neural induction by the secreted polypeptide noggin. Science 262: 713 -718. Launay C, Fromentoux V, Shi DL & Boucaut JC. (1996). A truncated FGF receptor blocks neural induction by endogenous Xenopus inducers. Development 122: 869 - 880. LeDouarin N. (1982). The neural crest. London: Cambridge University Press. Lee J, Hollenberg S, Snider L, Turner D, Lipnick N & Weintraub H. (1995). Conversion of Xenopus ectoderm into neurons by NeuroD, a basic helix-loop-helix protein. Science 268: 836-844. Liem K, Tremmi G, Roelink H & Jessell T. (1995). Dorsal differentiation of neural plate cells induced by BMP4-mediated signals from epidermal ectoderm. Cell 82: 969-979. Liem K, Tremml G & Jessel T. (1997). A role for the roof plate and its resident TGFrelated proteins in neuronal patterning in the dorsal spinal cord. Cell 91: 127-138. Logan MA, Steele MR, Van Raay TJ, Vetter ML. (2005). Identification of shared transcriptional targets for the proneural bHLH factors Xath5 and XNeuroD. Dev. Biol. 285: 570-583. Lönnerberg P, Schoenherr C, Anderson D & Ibáñez C. (1996). Cell Type-specific Regulation of Choline Acetyltransferase Gene Expression. J. Biol. Chem. 271: 33358– 33365. Lunyak V, Burgess R, Prefontaine G, Nelson C, Sze S, Chenoweth J, Shwartz P, Pevzner P, Glass C, Mandel G, and Rosenfeld M. (2002). Corepressor-Dependent Silencing of Chromosome Regions Encoding Neuronal Genes. Science 298: 1747- 1752. Luo T, Matsuo-Takasaki M and Sargent TD. (2001). Distinct roles for Distal-less genes Dlx3 and Dlx5 in regulating ectodermal development in Xenopus. Mol. Reprod. Dev. 60: 331-337.
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Page 1 and 2:
Universidad de Chile, Facultad de M
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Quisiera agradecer muy especialment
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Hipótesis……………………
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6. Función de XCoREST en el la esp
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Índice de figuras Figura 1. Experi
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Lista de publicaciones - Olguín P,
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embriones de Xenopus desde la blás
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laevis the ectoderm is patterned at
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Posteriormente, en otros vertebrado
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1.3 Inductores neurales y vías de
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formación de la placa neural y la
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Figura 3. Modelos de inducción neu
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destinos dependiendo de su localiza
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16 Figura 4. Modelo de doble gradie
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3.2 Neurogénesis primaria en Xenop
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adoptar el destino neural para fina
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neuronas ectópicas cuando es sobre
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Skarmeta et al., 1996) . Zic2 supri
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etrasan la diferenciación neuronal
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genes reporteros (ej. lacZ) que con
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manifiestan en la desacetilación d
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(Ballas et al., 2005) (Figura 11).
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corticales aislados y tratados con
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4.2.4 Función de REST/NRSF en neur
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1/NRSE mutado se expresa en gran pa
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silvestres. Sin embargo, en estadio
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tejido neural, como también en la
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participar en la neurogénesis a tr
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-Pinzas nº 5 (A. DUMONT y FILS, Su
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-MEMPFA (MOPS 0,1 M pH 7,0 EGTA 2 m
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-Bromuro de Etidio (GIBCO BRL, Cali
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concentración de mRNA a inyectar s
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Recolección de embriones. Para el
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Técnicas de Biología Molecular. A
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histona H3 fosforilada (Upstate) en
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Síntesis de ribosondas para hibrid
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Hibridación in situ (ISH). Los emb
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RESULTADOS 1. Clonamiento del cDNA
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Figura 14. Alineamiento de la secue
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3. Participación de XREST/NRSF en
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Figura 16. La interferencia de la f
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3.2 La interferencia de la función
Page 85 and 86: 74 Figura 17. La interferencia de l
Page 87 and 88: 4. Participación de XREST/NRSF en
Page 89 and 90: Figura 19. La interferencia de la f
Page 91 and 92: 4.2 La sobreexpresión de XREST/NRS
Page 93 and 94: 4.3 Los fenotipos ectodérmicos aso
Page 95 and 96: 4.4 La sobreexpresión de XREST1 no
Page 97 and 98: 4.5 La interferencia de la función
Page 99 and 100: 4.6 XREST/NRSF participa en la form
Page 101 and 102: Figura 24. Los fenotipos asociados
Page 103 and 104: Para poner a prueba esta idea se co
Page 105 and 106: 6. XCoREST participa en el establec
Page 107 and 108: Figura 26. XCoREST participa en el
Page 109 and 110: 1. XREST/NRSF regula la expresión
Page 111 and 112: 100 2. La interferencia de la funci
Page 113 and 114: 102 actividad de Wnt y FGF inducen
Page 115 and 116: 104 letalidad, probablemente porque
Page 117 and 118: 106 XREST/NRSF neuraliza los explan
Page 119 and 120: 108 mutada en los dominios de fosfo
Page 121 and 122: 110 5. XREST/NRSF modula la adquisi
Page 123 and 124: 112 cuenta de un potencial de acci
Page 125 and 126: 114 al neuroectodermo. La inhibici
Page 127 and 128: 116 embargo, estos resultados deben
Page 129 and 130: 118 Ballas N, Battaglioli E, Atouf
Page 131 and 132: 120 Chitnis AB. (1999). Control of
Page 133 and 134: 122 Godsave SF and Slack JM. (1989)
Page 135: 124 Kallunki P, Edelman GM & Jones
Page 139 and 140: 128 Oppenheimer JM. (1936b). Transp
Page 141 and 142: 130 Spemann H & Mangold H. (1924).
Page 143 and 144: 132 Wilson PA, Hemmati-Brivanlou A.
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Participación del Factor Silenciador Neuronal Restrictivo - Tesis ...

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