23.01.2015 Views

sodininkystė ir daržininkystė 25(4)

sodininkystė ir daržininkystė 25(4)

sodininkystė ir daržininkystė 25(4)

SHOW MORE
SHOW LESS

You also want an ePaper? Increase the reach of your titles

YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.

y than constant periods of cold. Although more complicated than the whole-plant<br />

method, this technique is best suited for the evaluation of freeze-thaw cycling as it<br />

is non-destructive and allows for continual sampling of the same tissues over<br />

multiple freeze-thaw cycles.<br />

Screening seedlings of Malus, 16–20 weeks after emergence, using a protocol<br />

to induce acclimation and select the hardiest individuals could improve the efficacy<br />

of breeding for cold hardiness by eliminating a large proportion of cold-tender seedlings<br />

before time and maintenance costs are invested in field plantings. Plant viability,<br />

collected in the form of binomial values and used to calculate percent seedling survival<br />

was a simple, efficient and precise method to segregate seedling populations for<br />

cold hardiness. We are presently running field trials with these same genotypes in the<br />

hopes of getting a test winter to compare our controlled studies to in vivo field<br />

results.<br />

Acknowledgements. Thanks to K. McRae and B. Walker for the<strong>ir</strong> statistical<br />

help and A. LeBlanc and M. Cao for the<strong>ir</strong> technical assistance.<br />

Gauta 2006-11-09<br />

Parengta spausdinti 2006-12-11<br />

References<br />

1. Caprio J. M. and Quamme H. A., Weather conditions associated with apple production<br />

in the Okanagan Valley of British Columbia. Can. J. Plant Sci. 1999. 79. P. 129–137.<br />

2. Kaye C. and Guy C. L. Perspective of plant cold tolerance; physiology and molecular<br />

responses. Sci. Prog. 1995. 78. P. 271–299.<br />

3. McCullagh P. and Nelder J. A. Generalized Linear Models, Chapman and Hall,<br />

London, 1983.<br />

4. Privé J.-P., Caprio J. M., Quamme H. A. and Embree C. Weather conditions associated<br />

with apple production in the Annapolis Valley of Nova Scotia and the Okanagan<br />

Valley of British Columbia. Proc. of the 11 th Atlantic Region Hydrotechnical Conference.<br />

2000. p. 33 (Abstr.)<br />

5. Privé J.-P., Embree C.G. Freezing media affects cooling rate and regrowth of KSC28<br />

apple rootstocks. Can. J. Plant Sci. 1997. 77. P. 461–471.<br />

6. Privé J.-P., LeBlanc A. Apple and small fruits research report. AAFC technical<br />

report no 99–01. 1999. 77 pp.<br />

7. Privé J.-P., Zhang M. I. N. Estimating cold stress in ‘Beautiful Arcade’ apple roots<br />

using electrical impedance analysis. HortTech. 1996. 6. P. 54–58.<br />

8. Repo T., Zhang M. I. N. Modelling woody plant tissues using a distributed electrical<br />

c<strong>ir</strong>cuit. J. Expt. Bot. 1993. 44. P. 977–982.<br />

9. Zhang M. I. N., Willison J. H. M. Electrical impedance analysis in plant tissues: In<br />

vivo detection of freezing injury. Can. J. Bot. 1992. 70. P. 2<strong>25</strong>4–2<strong>25</strong>8.<br />

10. Zhang M. I. N., Willison J. H. M., Xiao X. and Cheung C. H. Membrane-damage<br />

due to freeze-thaw stress in enhanced by post-thaw immersion in hypotonic solution.<br />

Can. J. Plant Sci. 1994. 74. P. 357–358.<br />

102

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!