- EPN products must be applied to moist soil; EPN’s can survive dry con<strong>di</strong>tions (Womersley, 1990) but cannot search for and locate the insect host. Product Opportunities - The market value; it is important that the market supports growing sales of the newly introduced product at a price that imme<strong>di</strong>ately provides a return on the investment of the initial product development. - No chemical available / phytotoxic effects/ chemical not effective; For example, in American cranberry crops no conventional chemical is available to control the Cranberry Girdler Chrysoteuchia topiaria, or strawberry root weevil Otiorhynchus ovatus (L). Growers were limited to using cultural methods to prevent pest damage until an EPN product was developed. In mushroom crops application of insecticides reduced yields. - EPN searching behaviour; in field grown crops it is particularly <strong>di</strong>fficult to apply insecticides at the point where the pest is causing damage. In strawberry plants grown under plastic access to the roots where black vine weevil larvae cause damage can be <strong>di</strong>fficult. However, by applying EPN’s through T-Tape irrigation systems infective juveniles introduced below the soil surface can search for and infect larvae developing in the strawberry roots. - Knowledge of the pest life cycle; expert knowledge obtained from universities and extension services can be used to determine the correct time for product application. It is important that the insect biology is understood to prevent ‘mysterious’ product failures. Product Threats Grower confidence; any change in the product can cause problems with grower confidence. Even a change in the colour of the product container can cause suspicions that something is not quite right with the production system. Product education; in the initial stages of product introduction <strong>di</strong>stributor and grower education is vital. If the growers misuse the product and observe damaged crops it is unlikely that they will continue to take risks on a ‘new’ idea. It is easy for a poor reputation to build up and almost impossible to recover confidence in the market. - Competitive conventional insecticides; the development of new conventional chemicals may address application problems, for example slow release formulations. References Akhurst, RJ and N. Boemare. 1990. Biology and taxonomy of Xenorhabus. In: Entomopathogenic nematodes in biological control R. Gaugler and H.K. Kaya, eds. CRC Press; Boca Raton FL. pp. 75-90. Boemare N., A. Givaudan, M. Brehélin and C. Laumond. 1997. Review article. Symbiosis and pathogenicity of nematode-bacterium complex. Symbiosis Vol. 22: 21-45 Gaugler, R. (1988). Ecological considerations in the biological control of soil-inhabiting insects with entomopathogenic nematodes. Agriculture, Ecosystems and Environment 24 351-360. Bowen D.J., T.A. Rocheleau, M. Blackburn, O. Andreev, E. Golubeva, R. Bhartia, and R.H. ffrench-Constant. 1998. Insecticidal toxin from the bacterium Photorhabdus luminescens. Science. 280. 2129-2132 Gaugler, R. and J. F. Campbell, 1991, Entomopathogenic nematode behavioural response to oxamyl, Ann. appl. Biol. 119, p. 131-138 Georgis, R., Kaya, H.K., and Gaugler, R. (1991). Effect of steinernematid and heterorhab<strong>di</strong>tid nematodes (Rhab<strong>di</strong>tida: Steinernematidae and Heterorhab<strong>di</strong>tidae) on non-target arthropods. Environ. Entomol. 20:815-822. Georgis, R. 1992. Present and future prospects for entomopathogenic nematode products. Biocontrol Science and Technology 2: 83-99. Huey, R. B., and E. R. Pianka, 1981, Ecological consequences of foraging mode, Ecology 62, 991-999. Ng K.K. and Webster J.M.. 1997. Antimycotic activity of Xenorhabdus bovienii (Enterobacteriaceae) metabolites against Phyotophthora infestans on potato plants. Can. J. Plant Pathology 19 (2): 125-136. Park S.H. & Paik S.U. 2001. Novel aliphatic amide having anticancer property. PCT Application WO01/49656 Poinar, G. O., Jr., 1990, Biology and Taxonomy of Steinernematidae and Heterorhab<strong>di</strong>tidae, In Entomopathogenic Nematodes in Biological Control, R. Gaugler and H. K. Kaya Eds, CRC Press, Boca Raton. pp. 23-58 Ragni A., Valentini F and Fridlender B.1997. “Insecticidal Bacteria”. PCTIL97/00246 Ricci M. and Fridlender B., New entomopathogenic nematodes active at low temperatures. PCT/IL99/00218 Simoes, N. 1994. Virulence factors produced by the entomopathogenic nematode Steinernema carpocapsae during parasitism. Proce. of the Vith Intern. Colloquium of Invertebrate Pathology Aug.28-Sept. 2. 1994. Montpellier, France p. 116-119 Vanninen, I., 1990, Depletion of endogenous lipid reserves in Steinernema feltiae and Heterorhab<strong>di</strong>tis bacteriophora and effect on infectivity, Procee<strong>di</strong>ngs and Abstracts of the 5th <strong>International</strong> Collogium on Invertebrate Pathology and Microbial Control, Adelaide. PDF creato con FinePrint pdfFactory versione <strong>di</strong>mostrativa http://www.secom.re.it/fineprint 36
Vinson, S. B., 1975, Biochemical coevolution between parasitoids and their hosts, In Evolutionary Strategies of Parasitic Insects and Mites, P. W. Price Ed., Academic Press, New York. Webster J.M & G. Chen. 1999. Dithiolpyrrolones and their correspon<strong>di</strong>ng and <strong>di</strong>oxides as antineoplastic agents. PCT Application WO99/12543. Womersley, C. Z., Dehydration Survival and Anhdrobiotic Potential, 1980. In Entomopathogenic Nematodes in Biological Control, R. Gaugler and H. K. Kaya Eds, CRC Press, Boca Raton. Wouts, W.M., 1980. Biology, life cycle and redescription of Neoaplectana bibionis Bovien, 1937 (Nematoda: Steinernematidae). J. Nematology 12 1 62-72. Zimmermann, G., 1996. Microbial control of vine weevil. Procee<strong>di</strong>ngs of the second international workshop on vine weevil (Otiorhynchus sulcatus Fabr.) (Coleoptera, Curculionidae). Braunschweig, Germany, May 21-23, 1996. PDF creato con FinePrint pdfFactory versione <strong>di</strong>mostrativa http://www.secom.re.it/fineprint 37
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15 ORGANIC WHEAT QUALITY AND PRODUC
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16 IL CONTROLLO DELLA “ MOSCA DEL
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Nel primo anno si può notare un an
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17 Il contenimento della ticchiolat
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Obtained recoveries percent were: 8
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[Pb] (ng/g) 250,00 200,00 150,00 10
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12. Agullo, G.; Gamet, L.; Besson,
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Tab. 3 Flavonol content (µg/ml) in
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mg/L Fig.3 3,50 3,00 2,50 2,00 1,50
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21 CONTAMINAZIONE DA MICOTOSSINE IN
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Ricerca: Aflatossine B1 B1+B2+G1+G2
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Campioni non Conformi 3,4 % B1 Camp
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25 EFFICACY EVALUATION OF BIOLOGICA
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Fig. 1 - Effects of Biological Cont
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26 IL CONTROLLO DELLE OPERAZIONI DI
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Results and Discussion S. carpocaps
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entompathogenic nematodes. CSC-EC E
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FIGURE 3. Insecticidal activity of
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Several colonies of Lepidopteran, C
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M. Scribano research work was suppo
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Nematode isolate % Mortality 3 100
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L’ultima parte della ricerca è s
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33 INVESTIGATIONS ON THE BACTERICID
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Vengono qui riportati i risultati d
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while for dinocap is 10 pg/µL. For
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[Pb](ng/ml) Figure 1: Pb content of