International Congress BIOLOGICAL PRODUCTS - Gruppo di ...

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METHODS FOR EVALUATION, IN CONTROLLED CONDITIONS, OF THE GROWTH OF OILSEED
RAPE PROMOTED BY BACTERIA
F. Citarrei, M. Scribano, S. Coranelli, C. Cellerino, L. Quattrocchi, L. Concezzi, and A. Ragni
BioTecnologie BT srl Pantalla di Todi 06050 Perugia Italy
Tel: ++39 – 075 - 895091 Fax: ++39 – 075 – 888 776
E-mail: aragni.bt@parco3a.org
Abstract
Growth promoters are microorganisms able to increase growth and productivity of plants by: atmospheric nitrogen
fixation, mineral salts solubilization and siderophore synthesis (ferrous natural chelants), production of substances
similar to plants hormones and with antagonistic effect towards phytopathogenic microorganisms. Growth promoters
can influence the availability of nutritive elements or they can improve the phytosanitary conditions of the plants. In the
present work we have tested the ability of some bacteria strain to promote the growth of oilseed rape [Brassica napus
(DC) Metzger var. oleifera, Brassiceae] by using two types of experimentations: in vitro, in laboratory and in vivo, in
greenhouse. The in vitro test was conducted with sterile seedlings monoxenically associated with bacteria isolates. In
the in vivo trials, the seeds were treated with bacteria prior to sawing. Both methods are able to select and evaluate the
promoting action of the bacteria towards the oilseed rape although the in vitro is faster and discriminate better the
promoting effect of the bacteria. In vitro test
Objective
To test the ability of some bacteria strains to promote the rapeseed growth in vitro
Materials and Methods
Sterile rape seeds were sown on MS/2 culture media; sterile seedlings were transplanted in MS culture media inoculated
with Pseudomonas sp. strain MK280 (Fig.1). This strain is able to produce siderophores that, as natural chelants, make
iron available for plants. The treatments in the Pseudomonas test are reported in Table 1.The following growth
parameters were checked at 15 and 30 days: plant height, plant fresh weight and plant dry weight. Table 1.
Experimental conditions of rape seed in vitro test with Pseudomonas sp. MK280
Codes Treatment description
FeCl3+MK280 Ferric chloride + bacteria MK280
FeCl3
Ferric chloride without bacteria MK280
Fe - Without iron, without bacteria MK280
FeEDTA+MK280 Chelated iron + bacteria
FeEDTA Chelated iron without bacteria MK280
Figure 1. Sterile seedlings of oilseed rape rapes transplanted
in MS culture media in a plant cell culture vessel.
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