International Congress BIOLOGICAL PRODUCTS - Gruppo di ...

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Table 1. Effectiveness against Sphaerotheca fusca of natural compounds tested in greenhouse and in field (a) . Compounds Rates Trials (%) Glasshouse Field Pinolene 1 +++ +++ Fresh milk 100 +++ Dried milk 10 +++ +++ Mineral oil 1 +++ +++ Sodium bicarbonate (NaHCO3) 0.5 +++ +++ Mineral oil + sodium bicarbonate 1+0.5 +++ +++ Mineral oil + sodium silicate 1+0.5 +++ +++ Potassium phosphate dibasic (K2HPO4) 0.5 +++ ++ Sodium phosphate dibasic (Na2HPO4) 0.5 +++ Ammonium phosphate dibasic [(NH4)2HPO4)] 1 +++ Calcium chloride (CaCl2) 0.5-1 +++ Magnesium chloride (MgCl2) 1 +++ Sodium silicate (Na2O . 2SiO2) 0.5 ++ Sodium hydroxide (NaOH) 0.25 ++ Ammonium phosphate monobasic (NH4H2PO4) 1 ++ Tripotassium phosphate (K3PO4) 0.5 ++ Trisodium phosphate (Na3PO4) 0.5 ++ Potassium phosphate monobasic (KH2PO4) 1 + Sodium phosphate monobasic (NaH2PO4) 0.5 + Potassium chloride (KCl) 1 + Sodium chloride (NaCl) 0.3-0.5 + Calcium carbonate (CaCO3) 1 + Calcium sulphate (CaSO4) 1 + Calcium silicate (CaO . 2SiO2) 1.5-2 + (a) +++ effective; ++ moderately effective; + poorly effective. The results herein discussed show that several compounds are effective in the early stages of infection or under low disease pressure, when applied repeatedly at short time intervals. Their effectiveness against powdery mildew proved to be influenced by temperature, time of application and especially by the uniformity of distribution on plants. In fact, field trials showed that one of their most important limits is the lack of control of powdery mildew on the lower surface of leaves. Cucurbits and other crops requiring sprays during a long harvesting time would benefit greatly from the usage of natural substances in crop protection. Acknowledgements: Work supported by the Italian Ministry of Agricultural and Forestry Policies in the frame of the research project “Technical tools for crop protection in organic agriculture” – Sub-project “Natural substances in crop protection from fungal diseases”. PDF creato con FinePrint pdfFactory versione dimostrativa http://www.secom.re.it/fineprint 60
8 METHODS FOR EVALUATION, IN CONTROLLED CONDITIONS, OF THE GROWTH OF OILSEED RAPE PROMOTED BY BACTERIA F. Citarrei, M. Scribano, S. Coranelli, C. Cellerino, L. Quattrocchi, L. Concezzi, and A. Ragni BioTecnologie BT srl Pantalla di Todi 06050 Perugia Italy Tel: ++39 – 075 - 895091 Fax: ++39 – 075 – 888 776 E-mail: aragni.bt@parco3a.org Abstract Growth promoters are microorganisms able to increase growth and productivity of plants by: atmospheric nitrogen fixation, mineral salts solubilization and siderophore synthesis (ferrous natural chelants), production of substances similar to plants hormones and with antagonistic effect towards phytopathogenic microorganisms. Growth promoters can influence the availability of nutritive elements or they can improve the phytosanitary conditions of the plants. In the present work we have tested the ability of some bacteria strain to promote the growth of oilseed rape [Brassica napus (DC) Metzger var. oleifera, Brassiceae] by using two types of experimentations: in vitro, in laboratory and in vivo, in greenhouse. The in vitro test was conducted with sterile seedlings monoxenically associated with bacteria isolates. In the in vivo trials, the seeds were treated with bacteria prior to sawing. Both methods are able to select and evaluate the promoting action of the bacteria towards the oilseed rape although the in vitro is faster and discriminate better the promoting effect of the bacteria. In vitro test Objective To test the ability of some bacteria strains to promote the rapeseed growth in vitro Materials and Methods Sterile rape seeds were sown on MS/2 culture media; sterile seedlings were transplanted in MS culture media inoculated with Pseudomonas sp. strain MK280 (Fig.1). This strain is able to produce siderophores that, as natural chelants, make iron available for plants. The treatments in the Pseudomonas test are reported in Table 1.The following growth parameters were checked at 15 and 30 days: plant height, plant fresh weight and plant dry weight. Table 1. Experimental conditions of rape seed in vitro test with Pseudomonas sp. MK280 Codes Treatment description FeCl3+MK280 Ferric chloride + bacteria MK280 FeCl3 Ferric chloride without bacteria MK280 Fe - Without iron, without bacteria MK280 FeEDTA+MK280 Chelated iron + bacteria FeEDTA Chelated iron without bacteria MK280 Figure 1. Sterile seedlings of oilseed rape rapes transplanted in MS culture media in a plant cell culture vessel. PDF creato con FinePrint pdfFactory versione dimostrativa http://www.secom.re.it/fineprint 61
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International Congress BIOLOGICAL P
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15:40-16:20 G. Lozzia Università d
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MAIN SPEAKERS • V. Rotondo FIAO,
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Il settore oggi è regolamentato in
Page 9 and 10: target botanical synthetic sodium c
Page 11 and 12: RELIABILITY AND PROSPECTIVES FOR TH
Page 13 and 14: Vineyard Passive diffusion Spontane
Page 15 and 16: of the host, a relatively limited n
Page 17 and 18: LOZZIA G.C., RIGAMONTI I.E., 1994 -
Page 19 and 20: DIFESA DELLE COLTURE DAI PATOGENI F
Page 21 and 22: THE FUNGAL PHYTOTOXINS AND THEIR PO
Page 23 and 24: NEED FOR RESEARCH AND APPROPRIATE A
Page 25 and 26: PRESENCE OF OCHRATOXIN IN EXPERIMEN
Page 27 and 28: 5. Hohler, D. (1998). Ochratoxin A
Page 29 and 30: Table 3: Pesticide treatments on
Page 31 and 32: BIOPESTICIDES: EVALUATION PROCESS F
Page 33 and 34: Xenorhabdus spp. and Photorhabdus s
Page 35 and 36: It can be reproduced in vivo at 15
Page 37 and 38: Vinson, S. B., 1975, Biochemical co
Page 39 and 40: PROTECTION OF GRAPEVINE FROM POWDER
Page 41 and 42: I MEZZI TECNICI PER L'AGRICOLTURA B
Page 43 and 44: POSTER 1. Agosteo G.E., Ambrosio M.
Page 45 and 46: 31. Pietri A., Bertuzzi T., Barbier
Page 47 and 48: 1 PROVE DI LOTTA ALL’OIDIO DELLO
Page 49 and 50: 2 LUPINUS ALBUS, AN ANCIENT EUROPEA
Page 51 and 52: Table 1. Benzo(a)pyrene and benzo(e
Page 53 and 54: 5 INSETTICIDI D’ORIGINE VEGETALE
Page 55 and 56: Tabella 2. Piretrine: composizione
Page 57 and 58: californicus (fitoseide predatore)
Page 59: 7 NATURAL COMPOUNDS IN THE CONTROL
Page 63 and 64: Figure 3. Effect of Pseudomonas sp.
Page 65 and 66: 9 IMPROVEMENT OF SOIL PROPERTIES AN
Page 67 and 68: (mg kg-1) (mg kg-1) 140 120 100 80
Page 69 and 70: 10 NEMATICIDAL ACTIVITY OF AQUEOUS
Page 71 and 72: % cumulative hatch 40 35 30 25 20 1
Page 73 and 74: ROTENONE Il principio attivo è est
Page 75 and 76: - azadiractina nei confronti di F.
Page 77 and 78: Recovery and repeatabilty tests wer
Page 79 and 80: 13 IMPIEGO DEL TIMOLO NEL CONTROLLO
Page 81 and 82: dello standard esterno. E’ stata
Page 83 and 84: Tab. III - Residui di timolo(mg/Kg)
Page 85 and 86: phytotoxicity index (%) Results In
Page 87 and 88: 15 ORGANIC WHEAT QUALITY AND PRODUC
Page 89 and 90: 16 IL CONTROLLO DELLA “ MOSCA DEL
Page 91 and 92: Nel primo anno si può notare un an
Page 93 and 94: 17 Il contenimento della ticchiolat
Page 95 and 96: Obtained recoveries percent were: 8
Page 97 and 98: [Pb] (ng/g) 250,00 200,00 150,00 10
Page 99 and 100: Supelco Supelclean LC-18 SPE tubes
Page 101 and 102: 12. Agullo, G.; Gamet, L.; Besson,
Page 103 and 104: Tab. 3 Flavonol content (µg/ml) in
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Page 107 and 108: Table 1 - Fungicides used during th
Page 109 and 110: Table 3 - Efficacy of different tre
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21 CONTAMINAZIONE DA MICOTOSSINE IN
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Ricerca: Aflatossine B1 B1+B2+G1+G2
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Campioni non Conformi 3,4 % B1 Camp
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22 PEPTIDATI DI RAME: PRODOTTI INNO
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23 CHARACTERIZATION OF ORGANIC VIRG
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24 CHARACTERIZATION OF ORGANIC VIRG
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25 EFFICACY EVALUATION OF BIOLOGICA
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Fig. 1 - Effects of Biological Cont
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26 IL CONTROLLO DELLE OPERAZIONI DI
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Results and Discussion S. carpocaps
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entompathogenic nematodes. CSC-EC E
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After this preliminary screening an
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FIGURE 3. Insecticidal activity of
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Several colonies of Lepidopteran, C
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PDF creato con FinePrint pdfFactory
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M. Scribano research work was suppo
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Nematode isolate % Mortality 3 100
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In our trial organic wheat showed a
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per "strippare" i vapori nitrosi re
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L’ultima parte della ricerca è s
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33 INVESTIGATIONS ON THE BACTERICID
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Vengono qui riportati i risultati d
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while for dinocap is 10 pg/µL. For
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[Pb](ng/ml) Figure 1: Pb content of
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