International Congress BIOLOGICAL PRODUCTS - Gruppo di ...

Figure 3. Effect of Pseudomonas sp. MK280 on rapeseed in vitro growth: plant height
cm
25
20
15
10
5
0
FeCl3 + MK280 FeCl3 FeEDTA + MK280 FeEDTA Fe -
Treatments
63
plant height 15 days
plant height 30 days
Figure 4. Effect of Pseudomonas sp. MK280 on rapeseed in vitro growth: plant fresh and dry weight
Greenhouse trial
g
1,2
1
0,8
0,6
0,4
0,2
0
FeCl3 +
MK280
FeCl3 FeEDTA +
MK280
Treatments
fresh weight 15 days
fresh weight 30 days
dry weight 15 days
dry weight 30 days
FeEDTA Fe -
Objective
To test the ability of some bacteria strains to promote the rapeseed growth in controlled conditions.
Materials and Methods
The trial was conducted using the following bacteria: Azospirillum brasilense (strain LMD78.36) or Pseudomonas sp.
(strains MK280, Hv37a and AGS195B). The seeds were treated with bacteria strain cell suspensions; then the treated
seeds were sown in a pot (one seed/pot) containing natural soil (Nitrogen= 0.08%; Organic matter =1.2%; Iron = 10.77
mg kg -1 )The strains were tested on 20 plants, 10 plants were fertilized with Nitrogen (60 kg/ha) after 40 days from
sowing; 10 were not fertilized. The following growth parameters were checked at 15 and 30 days: plant height, plant
fresh weight and plant dry weight.
Table 2. Colony Forming Unit from rape seeds treated with bacteria before sowing in greenhouse.
Bacteria C.F.U/seed
A. brasiliense LMD78.36 6 x 10 3
Pseudomonas sp. MK280 1.2 x 10 5
Pseudomonas sp. HV37a 6 x 10 5
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