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Tecnai on-line help manual -- Options - UT Southwestern

Tecnai on-line help manual -- Options - UT Southwestern

Tecnai on-line help manual -- Options - UT Southwestern

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<str<strong>on</strong>g>Tecnai</str<strong>on</strong>g> <strong>on</strong>-<strong>line</strong> <strong>help</strong> Opti<strong>on</strong>s 105Opti<strong>on</strong>s Software versi<strong>on</strong> 2Camera c<strong>on</strong>stantThe magnificati<strong>on</strong> of the stereographic projecti<strong>on</strong> and the other elements displayed depends <strong>on</strong> thecamera c<strong>on</strong>stant. This value can either be defined by hand, using the camera c<strong>on</strong>stant slider, orautomatic (in which case it is linked to the active camera length). The range of camera c<strong>on</strong>stants usedby k-Space C<strong>on</strong>trol is 1 to 20.The camera c<strong>on</strong>stant (the camera length*the electr<strong>on</strong> wavelength) is in mm*nm, so a camera c<strong>on</strong>stant of1 means a distance of 1 mm corresp<strong>on</strong>ds to 1 nm d spacing (<strong>on</strong> the printed page; due to m<strong>on</strong>itor sizedifference these values may not be exact <strong>on</strong> the m<strong>on</strong>itor display).The camera c<strong>on</strong>stant (L*λ) forms part of the formula for c<strong>on</strong>verting distances in a diffracti<strong>on</strong> pattern to dspacings:D*d = L*λ or d = (L*λ ) / Dwhere D is the distance of the diffracti<strong>on</strong> spot in the pattern to the origin of the pattern (the transmittedbeam). Thus while 1 mm at a camera c<strong>on</strong>stant of 1 means 1 nm d spacing, 2 mm means 0.5 nm dspacing (as D occurs in the denominator).Table of (approximate) camera lengths against camera c<strong>on</strong>stant for different accelerating voltages.Cam. c<strong>on</strong>st. 120 kV 200 kV 300 kV1 300 mm 400 mm 500 mm5 1500 mm 2000 mm 2500 mm10 3000 mm 4000 mm 5000 mm20 6000 mm 8000 mm 10 000 mmNote: For displaying the diffracti<strong>on</strong> pattern, it is necessary to choose a larger camera c<strong>on</strong>stant,otherwise the spots will be too close together and invisible. If the diffracti<strong>on</strong> pattern display is <strong>on</strong> and nopattern is visible, increase the camera c<strong>on</strong>stant.The display at the smallest camerac<strong>on</strong>stant.

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