Tecnai on-line help manual -- Options - UT Southwestern

<strong>Tecnai</strong> on-line help Options 12Options Software version 21.2.1 Expose panelExpose buttonWhen the Expose button (or the <strong>Tecnai</strong> Exposure button on the left-hand Control Pad) is pressed, theactual Low Dose exposure is started. The progress of the exposure is displayed in the status as well asby the status of the state and blank buttons. The medium for exposure (plate camera or CCD) dependson the setting chosen.SeriesThe Series check box enables the automatic exposure of a series. The settings for the series are definedin the Series panel. The Series check box is only enabled when at least one setting has been defined forseries.DoubleThe Double check box enables double exposure (multiple exposures on a single plate). The exposuretimes for the double exposures are defined in the Double panel. The Double check box is only enabledwhen at least one setting has been defined for double. Double and Series or Use spotscan cannot beused together.Dim screenIn order to prevent light from the monitor from striking the plate (which is lying open in the projectionchamber during the exposure; it is therefore always important to switch room lights off and cover thewindows of the projection chamber), the monitor screen can be blanked during the exposure.Note: Should the screen dimmer fail to be removed after the exposure, then it can always be removed bypressing Shift+Esc on the keyboard. Do not use Alt+Tab to switch to another window because that will(in most cases) not work.Use spotscanOnly on systems where spotscan is installed :When the Use spotscan option is enabled the exposure(s) will be taken as spotscans, not flood-beamexposures. The Exposure time will change to spot dwell time (exposure time per spot). The spotscan asexecuted is defined in the Spotscan tab of the flap-out. Spotscan and pre-exposure or Double cannot becombined.Exposure timeBecause the conditions during operation (search and focus) are not suitable for measuring the exposuretime to be used during the actual Low Dose exposure (due to differing magnification/camera length, spotsize and intensity settings), the exposure time must be set by the operator. Switch to the Exposure stateinitially and determine the exposure time as indicated by the plate camera of the <strong>Tecnai</strong> microscope. If