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Tecnai on-line help manual -- Options - UT Southwestern

Tecnai on-line help manual -- Options - UT Southwestern

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<str<strong>on</strong>g>Tecnai</str<strong>on</strong>g> <strong>on</strong>-<strong>line</strong> <strong>help</strong> Opti<strong>on</strong>s 162Opti<strong>on</strong>s Software versi<strong>on</strong> 212.3 MRC file formatThe MRC file is a file with extensi<strong>on</strong> .mrc. It c<strong>on</strong>tains a primary header and an extended header asdescribed below, followed by the data. The file format is derived from the MRC file format which wasoriginated at the Medical Research Council in Cambridge, England.• The MRC format can c<strong>on</strong>tain a series of images in a single file. The format c<strong>on</strong>sists of:• A primary header. A 1024 byte secti<strong>on</strong>.• An extended header. Additi<strong>on</strong>al informati<strong>on</strong> about the individual images.• Image data.12.3.1 The primary header.Data type Name Byte Commentpositi<strong>on</strong>integer (4-byte) nx 0 number of columsinteger ny 4 number of rowsinteger nz 8 number of secti<strong>on</strong>s (i.e. images)integer mode 12 data type, 1 = pixel value stored as short integerinteger nxstart 16 lower bound of columsinteger nystart 20 lower bound of rowsinteger nzstart 24 lower bound of secti<strong>on</strong>sinteger mx 28 cell size in Angstroms (pixel spacing=xlen/mx)integer my 32integer mz 364-byte floating point xlen 404-byte floating point ylen 444-byte floating point zlen 484-byte floating point alpha 52 cell angles in degrees4-byte floating point beta 564-byte floating point gamma 60integer mapc 64 mapping colums, rows, secti<strong>on</strong>s <strong>on</strong> axis(x=1,y=2,z=3)integer mapr 68integer maps 724-byte floating point amin 764-byte floating point amax 804-byte floating point amean 842-byte integer ispg 88 space group number (0 for images)2-byte integer nsymbt90 number of bytesused for storingsymmetry operators4-byte integer next 92 number of bytes in extended header. This is animportant number. It defines the offset to the firstimage2-byte integer dvid 96 creator idchar (byte) extra[30] 98 extra 30 bytes data (not used)2-byte integer numintegers 128 number of bytes per secti<strong>on</strong> in extended header2-byte integer numfloats 130 number of floats per secti<strong>on</strong> in extended header2-byte integer sub 1322-byte integer zfac 134

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