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Tecnai on-line help manual -- Options - UT Southwestern

Tecnai on-line help manual -- Options - UT Southwestern

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<str<strong>on</strong>g>Tecnai</str<strong>on</strong>g> <strong>on</strong>-<strong>line</strong> <strong>help</strong> Opti<strong>on</strong>s 50Opti<strong>on</strong>s Software versi<strong>on</strong> 23.16.10 Help menuThe Help menu c<strong>on</strong>tains two items, Show and About. Selecting Show (or pressing F1) will display thisHelp file in the <str<strong>on</strong>g>Tecnai</str<strong>on</strong>g> User Interface. About will display the About TEM Photom<strong>on</strong>tage dialog listing thecurrent versi<strong>on</strong> number of the software. When reporting bugs, always include the versi<strong>on</strong> number in thereport.3.17 C<strong>on</strong>trol opti<strong>on</strong>sThe software supports a range of opti<strong>on</strong>s, defined in the C<strong>on</strong>trol opti<strong>on</strong>s dialog. Opti<strong>on</strong>s are stored asset for each user separately and recalled up<strong>on</strong> restart. Opti<strong>on</strong>s can be saved to file and reloaded.Plate typeNot relevant for CCD m<strong>on</strong>tage.CCDWith CCD acquisiti<strong>on</strong>, there are two ways to handle the images:• Collect the images into a data series in TIA. This series can then be used later as the basis forgenerating the m<strong>on</strong>tage image. The number of images str<strong>on</strong>gly depends <strong>on</strong> image size and memoryavailable.• Collect the images as a file series. Each image is saved to file but not kept in a data series in TIA.While there is no limit to the number of images (except hard-disk space), you have to use othersoftware to assemble the images into a single m<strong>on</strong>tage image.• The CCD Store images <strong>on</strong>ly is disabled if you have already acquired images. To switch betweendata series in TIA and files, you have to redefine the m<strong>on</strong>tage (File, New m<strong>on</strong>tage).OverlapThe overlap between the exposures is the percentage of the horiz<strong>on</strong>tal or vertical axis that must becomm<strong>on</strong> to both exposures to ensure fit of the photom<strong>on</strong>tage without gaps. The overlap can be seteither automatically or at a fixed value. If d<strong>on</strong>e automatically the program will adjust the overlap for themagnificati<strong>on</strong> used.Note: It is allowed to define a negative fixed overlap value. In that case the individual exposures will notoverlap but instead be spaced apart. In this way an area can be covered by a regular grid of exposureswithout requiring them to fill the whole area. This functi<strong>on</strong> can be used for example for statisticalanalysis.

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