Tecnai on-line help manual -- Options - UT Southwestern

<strong>Tecnai</strong> on-line help Options 158Options Software version 2Stage calibrationThe stage calibration calibrates the stage x and y shifts. This calibration is needed for consistencychecks of the eucentric height procedure.Stigmator alignmentThe stigmator alignment ensures that image shifts caused by changes of the stigmator (objective in HMimage and Nanoprobe; diffraction in LM image) are minimised. This procedure performs the same as thestigmator alignment procedures themselves (in the Alignment Control Panel) except in AutoAdjust thealignment is done automatically. It should be noted that the focus/stigmator calibration is dependent onthis alignment and a change in the alignment will interfere with proper operation of the AutoAdjustfunctions.Focus/Stigmator calibrationThe focus/stigmator calibration procedure performs the calibration of beam tilt, focus/stigmator andimage shift. Once this calibration has been done, the focus/procedure can be run for any magnificationthat is smaller than ~150kx (above the beam-tilt induced image-shift method doesn't work anymorebecause random fluctuations of image position start to play an important role and interfere too much withthe focus/astigmatism measurement).