Tecnai on-line help manual -- Options - UT Southwestern

<strong>Tecnai</strong> on-line help Options 118Options Software version 2tension setting. If TIA CCD is present on a system with Gatan cameras, the software will also requirethat TIA is running (otherwise the TIA CCD magnification table will not be set).The Start button will change to a yellow color and its caption toStop. Pressing the Start button when it is yellow will stop thecalibration procedure.Starting the calibration procedure will open a log in a separate window. The log shows what is beingdone and what the results are. It can be saved in text format (under the File menu of the log window).The log can also be printed (if a printer is available to the system). Text files can also be opened in thelog file window.Next buttonDuring the calibration procedure the operator will be expected to do certain things as setting upillumination, specimen area and focus, as well as change specimen (later in the procedure). Once thesethings have been done (as given by the instructions displayed), pressing the Next button will continuewith the procedure.Flap-out buttonThe flap-out button leads to the Report, File and Options tabs of the Automated Experiments ControlPanel.9.1.1 The HM magnification calibration procedureThe calibrations procedure consists of a calibration for each magnification of the series. The followingprocedure is used:• Direct cross-grating calibration of the lowermost SA magnification.• Calibration of the image shift at the lowermost SA magnification (this does not calibrate amagnification, but the image shift itself, used later).• Calibration of the Mi magnifications with the cross-grating method.• Calibration of the remaining SA magnifications, going up in magnification. The software detects themagnification at which the cross-grating method does not work anymore and displays a warning inthat sense. Then the software automatically switches to the image shift method. Once themagnification is high enough for high-resolution images (determined from the measured pixel size),the software will switch to the lattice-image method.• Calibration of the Mh magnifications with the lattice-image method.