<str<strong>on</strong>g>Tecnai</str<strong>on</strong>g> <strong>on</strong>-<strong>line</strong> <strong>help</strong> Opti<strong>on</strong>s 26Opti<strong>on</strong>s Software versi<strong>on</strong> 21.7 TV / CCD tabThe TV / CCD tab of the Low Dose C<strong>on</strong>trol Panel c<strong>on</strong>tains the informati<strong>on</strong> that Low Dose uses withregard to cameras (TV and CCD). The system works in combinati<strong>on</strong> with the settings defined for theSearch, Focus and Exposure states. In some cases Low Dose automatically detects what cameras are<strong>on</strong> the system, while in other cases the user has to add some. The following are detected automatically:• If an off-axis TV is present in the microscope software c<strong>on</strong>figurati<strong>on</strong>, the off-axis TV-rate camera boxis always checked (the user cannot remove the check mark). The same applies to the "TV-ratecamera present check box".• If a Gatan Imaging Filter is present, the GIF TV camera (retractable) is assumed to the present andthe box (bottom left) is checked. Once again, the user cannot remove this check.• All CCD cameras are automatically detected and their locati<strong>on</strong> is either retrieved from the storedmicroscope c<strong>on</strong>figurati<strong>on</strong> (e.g. Tietz cameras) or from their name (e.g. Gatan). In the case when thenaming of a camera is possible, the names must be as follows: if the CCD camera is in the wideangleport (above the projecti<strong>on</strong> chamber), the name must have the character sequence "WA"; if thecamera is that of a GIF (Imaging Filter), the character sequence "GIF" must be in the name; if it is anoff-axis CCD, the character sequence "off-axis" must be present in the name. Upper- or lowercasedoes not matter. All cameras not having the above character sequences are assumed to be <strong>on</strong> <strong>on</strong>axisbelow the projecti<strong>on</strong> chamber. Cameras identified are indicated by check marks in the boxes <strong>on</strong>the right-hand side of the schematic microscope picture. Once again, these check marks cannot bechanged.
<str<strong>on</strong>g>Tecnai</str<strong>on</strong>g> <strong>on</strong>-<strong>line</strong> <strong>help</strong> Opti<strong>on</strong>s 27Opti<strong>on</strong>s Software versi<strong>on</strong> 21.7.1 Low Dose State mediaBecause CCD cameras are not always accessible (depending <strong>on</strong> whether the c<strong>on</strong>trolling software isrunning and/or the camera is switched <strong>on</strong>), the system used for selecting the media for the different LowDose states has been set up as flexibly as possible. The accessible media are always shown in themedia lists <strong>on</strong> the Settings tab. There are potentially ten media:• Screen - for Search and Focus• Plate - for Exposure• Four different TV positi<strong>on</strong>s - for Search and Focus• Four different CCD positi<strong>on</strong>s - for all statesA medium is identified by its positi<strong>on</strong>, not its name. Thus, if you can access a particular CCD throughdifferent c<strong>on</strong>trollers, the medium will be chosen <strong>on</strong> the basis of where it is located, even if you changec<strong>on</strong>troller. If a medium is not accessible (e.g. a CCD c<strong>on</strong>troller has not been started or shut down), it willdisappear from the lists. The selecti<strong>on</strong> in the list will temporarily default to screen or plate. When themedium becomes accessible, it will re-appear in the lists and the selecti<strong>on</strong> will change back to themedium as before - unless the user changes the medium selecti<strong>on</strong> in the list.The same thing logic applies to the selecti<strong>on</strong> when loading settings files. If the medium is available, it willbecome selected. If not, it will <strong>on</strong>ly become selected <strong>on</strong>ce the medium is available and the defaultmedium is selected in the meantime). Note that what is saved in the settings files are the media thatwould be selected when the proper CCD c<strong>on</strong>troller(s) is(are) present, and not necessarily what iscurrently displayed in the lists as chosen.An exampleIn the example we have a system with an Imaging Filter plus an off-axis TV camera. The user sets upthe system such that:• Search is <strong>on</strong> the off-axis TV camera. Because the off-axis shift in EFTEM is too limited at lowmagnificati<strong>on</strong>s, the Search magnificati<strong>on</strong> is chosen sufficiently high that the off-axis image shift canreach the camera (note that Low Dose does not change lens series - normal to and EFTEM or viceversa).• Focus is defined <strong>on</strong> the GIF TV-rate camera.• Exposure is <strong>on</strong> the GIF CCD camera.When changing states, Low Dose will automatically select the appropriate detector positi<strong>on</strong>, lift theviewing screen, and insert or retract the GIF TV camera. When DigitalMicrograph is not available, themedia will default to off-axis TV (since this does not rely <strong>on</strong> DigitalMicrograph), screen (the GIF TVcamera c<strong>on</strong>trol is <strong>on</strong>ly through DigitalMicrograph) and plate (the CCD camera is not available). IfDigitalMicrograph becomes available again, the media will automatically be switched back to theprevious selecti<strong>on</strong>.TV-rate camera presentIf a TV-rate camera is present, it can be used in the Search and Focus states. Whenever possible, theLow Dose software will <strong>help</strong> in using the TV-rate camera, either by reminding the user to insert thecamera or by automatically lifting the screen (when the TV-rate camera is located underneath theviewing screen) and/or inserting the camera.CCD camera c<strong>on</strong>trollerIf a CCD camera is present, it can be used in the Search and Focus state for viewing and in theExposure state for recording. In all cases the Search, Focus and Acquire setups must be d<strong>on</strong>e in the<str<strong>on</strong>g>Tecnai</str<strong>on</strong>g> CCD/TV C<strong>on</strong>trol Panel. Low dose simply uses the predefined setups (Search = Search, Focus =Preview, Exposure = Acquire) and does not change any settings (except the exposure time used for theLow Dose Exposure). If more than <strong>on</strong>e c<strong>on</strong>troller is present, you can change the c<strong>on</strong>troller to be used byselecting <strong>on</strong>e from the drop-down list (this functi<strong>on</strong> is identical to that in the CCD/TV C<strong>on</strong>trol Panel). Thec<strong>on</strong>troller is not specific to Low Dose. Low Dose does not automatically select a c<strong>on</strong>troller.