Tecnai on-line help manual -- Options - UT Southwestern

<strong>Tecnai</strong> on-line help Options 40Options Software version 23.8 Setting up for a montageThe setup for a montage follows these steps:• Define the boundary points for the montage area.• Define the magnification to be used.• If required, calibrate the rotation between the stage axes and the image axes.3.9 Define boundary pointsThe boundary points define the perimeter of the photomontage area. The perimeter can be any shapedefined by a polygon of up to 500 points. These points themselves are determined simply by moving thespecimen stage so a point on the boundary lies in the center of the viewing screen. After a minimum oftwo points have been loaded, the program will display a gray area that represents the photomontagearea (for two points it will be a line).One aspect is critical in determining the boundary points. They must follow the perimeter of thephotomontage in their proper sequence (starting at one point and going around until the same startingpoint is encountered again). If a point is out of sequence the program may display the photomontagearea with gaps as in the left-hand image below.Improper sequence.Point 4 from left figure removed.The strange shape of the area in the left-hand picture is the result of intersecting lines and does notproperly represent the photomontage because point 4 was loaded between points 3 and 5 instead ofbetween points 1 and 2. If a point is found to be out of sequence, then use the hyperlabel option todelete that point.3.10 Set magnificationSet magnification is the Setup step where the magnification for the photomontage is defined. Theoperator is asked to set the microscope magnification to the desired value, after which the program will