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Tecnai on-line help manual -- Options - UT Southwestern

Tecnai on-line help manual -- Options - UT Southwestern

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<str<strong>on</strong>g>Tecnai</str<strong>on</strong>g> <strong>on</strong>-<strong>line</strong> <strong>help</strong> Opti<strong>on</strong>s 66Opti<strong>on</strong>s Software versi<strong>on</strong> 2A Random scan is a scan where the software calculates grid-holepositi<strong>on</strong>s <strong>on</strong> the basis of random moves in the X,Y directi<strong>on</strong>. In this way itis possible to remove operator bias from the procedure and thus toreduce the number of grid holes that must be scanned for statisticalc<strong>on</strong>fidence.Each hole passed is based <strong>on</strong> a random selecti<strong>on</strong> of X and Y positi<strong>on</strong>.Movement from <strong>on</strong>e hole to the next is out<strong>line</strong>d by the red <strong>line</strong> andarrows.Scan typeThe Scan type can be either <strong>manual</strong> or automatic. In <strong>manual</strong> scan, the operator must command thesoftware to move the specimen stage from <strong>on</strong>e hole to the next. In automatic scan, the software waitsuntil the delay time has passed and then moves the stage to the next hole.Opti<strong>on</strong>sRelocatable gridA Relocatable grid is a specially shaped grid that has an asymmetric 'ear'that fits into the single-tilt specimen holders delivered since 1993. The 'ear'takes care that the grid can be loaded into the specimen in <strong>on</strong>ly <strong>on</strong>e way,without rotati<strong>on</strong> and very little sideways movement. The relocatable grid thusmakes it easy to remove specimens from the microscope holder and puttingthem back in and still retrieve stored locati<strong>on</strong>s easily.If relocatable grids are used, switch <strong>on</strong> the opti<strong>on</strong> under C<strong>on</strong>trol Opti<strong>on</strong>s.Grid Scanning will then make it possible to drive back directly to the oldlocati<strong>on</strong> of the first reference point which should be very close to the currentlocati<strong>on</strong> of that point.Use labelsThe label refers to user-written comments (up to a maximum of 100 characters) than are stored togetherwith stored locati<strong>on</strong>s. If Use labels is checked the software will bring up a dialog allowing inserti<strong>on</strong> of thelocati<strong>on</strong> label.Use hyperlabelsThe software provides a special feature in the form of hyperlabels (labels that act like hyperlinks: whenthe cursor moves over them they flash their color to blue and a mouse-click brings up a dialog withinformati<strong>on</strong> about the point, with additi<strong>on</strong>al functi<strong>on</strong>ality such as Go to or Delete).Use Functi<strong>on</strong> keysFuncti<strong>on</strong> keys can be used in additi<strong>on</strong> to menu commands to c<strong>on</strong>trol Grid Scanning. The followingfuncti<strong>on</strong>s are used:• Up, down, left, right arrows in cursor scan (these have no menu equivalent).• F4: Add locati<strong>on</strong>• F6: Next hole• F8: Previous hole

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