Tecnai on-line help manual -- Options - UT Southwestern

<strong>Tecnai</strong> on-line help Options 15Options Software version 2Delete buttonThe Delete button allows removal of settings from the list. Select a setting and press Delete.Move upThe through-focus exposures are recorded in the sequence as they occur in the list. To change thesequence, select a setting and press Move up to move it up in the list.Move downThe through-focus exposures are recorded in the sequence as they occur in the list. To change thesequence, select a setting and press Move down to move it down in the list.1.2.4 Double panelThe double panel contains settings used for recording doubleexposure (multiple exposures on a single plate). The doubleexposure setup is totally flexible. It simply will execute as manyexposures as there are entries in the list, changing the exposuretimes as indicated in the list for the particular setting.The double exposure sequence is similar to that for a normal low-dose exposure except that the plate isnot removed until the last double exposure has been done (or the exposure is cancelled). After oneexposure has been recorded, further execution pauses until the operator presses the Expose button (onthe Low Dose Control Panel) or the Exposure button (on the left-hand <strong>Tecnai</strong> Control Pad) again.Pressing either of these buttons while an exposure is taking place (the Expose button is yellow) cancelsfurther exposure, otherwise it starts the next exposure in the sequence. When dim screen is active, thedimmed screen will display a message alerting the operator to press Exposure to start the nextexposure.Between individual exposures of a double exposure sequence, it is possible to lower the screen and lookat the image. Be aware, however, that the Low Dose state remains in Exposure. The Peek and BeamBlanker functions become accessible when the screen is lowered.When Double exposure is selected in the Expose tab, the Low Dose exposure time as listed becomesmeaningless, and the double exposure times in the list are used instead.Exposure timeThe exposure times list is filled by entering values for the exposure time (in seconds) in the exposuretime edit control and pressing the Add button.Exposure times listThe exposure times list contains the list of values used for the double exposure times. The maximumnumber of values is 20.Add buttonThe Add button allows insertion of a new setting into the exposure times list. The value for the newexposure time must be inserted into the exposure time edit control.