Tecnai on-line help manual -- Options - UT Southwestern

<strong>Tecnai</strong> on-line help Options 41Options Software version 2read the value form the microscope. If no rotation calibration is necessary the software will proceed bycalculating the photomontage. First it quickly estimates the number of exposures required to cover themontage area. If the estimate indicates more exposures than the default plate stock of 56 exposures, awarning is given. The operator can then proceed or reset the magnification to a lower value.After the estimate the montage is calculated. Dependent on a variety of factors the suggested montagemay be good enough or it may require modification. In the former case you can proceed directly toautomatic exposure (plate camera only) of manual exposure.3.11 Calibrate rotationRotation calibration is the setup step wherein the program is informed about the rotation angle betweenthe specimen stage X,Y axes and the camera. This is done simply by moving a recognisable imagedetail from one screen marker on the left to one on the right .The Rotation calibration dialog during the first step. In thesecond step the feature selected is moved to the markeron the right-hand side.Once a rotation value has been determined for a particular magnification, it will be stored by thesoftware.3.12 Modifying a montageThe montage suggested by the software can be modified in various ways. In order to modify themontage, you have to switch modification on. During a modification procedure, the changes can beundone. The Undo will revert the montage to its configuration before modification was switched on. Youcan go through repeated cycles by switching modification on and off again.During modification mouse double-click and mouse click-and-drag operations on the window have thefollowing meaning.• Mouse double-click puts a cross marker at the point where the cursor was during the double click. Ifthe cross lies inside an exposure (don't go too far towards the edge; also don't click on the label if it isa hyperlabel, because then the hyperlabel dialog comes up) that exposure can be deleted. If thecross lies near a potential exposure location, that location can be inserted as a new exposure.• Mouse click-and-drag shifts the exposures relative to the montage area (the actual shift is onlydisplayed once the mouse button is released.