Tecnai on-line help manual -- Options - UT Southwestern

<strong>Tecnai</strong> on-line help Options 43Options Software version 2entered with 'Loc X xxx.xx Y yyy.yy' where xxx.xx and yyy.yy are replaced by the stage x,y location ofthe exposure. Both options can be used together. Existing film text will be shifted down (if more than 4lines exist after insertion of the montage data, the excess lines will be lost and not restored afterwards).After the exposure procedure is stopped, the film text is restored to its original setting (minus any excesslines lost).Exposures that have been recorded will have their plate exposure numbered entered in the list and, ifsufficient space is available, their label on the screen is also replaced by the exposure number.Note 1: The procedure will stop or pause when an error is encountered, e.g. plate stock zero. In thatcase the operator can rectify the condition leading to the error and continuing with the procedure.Note 2: While exposing (both automatic and manual), the program uses a backlash correction procedureto make the positioning the exposures more accurate. However, because the backlash correction(probably) wasn't done during definition of the boundaries, there may be a systematic shift of the wholepattern of exposures of up to about 500 nm. For montages at high magnifications it is important thereforeto check the exposure locations (e.g. go to selected exposure locations through hyperlabels), especiallyif the edge of the area covered by the exposures is close (less than 1 um) to the intended photomontagearea.3.14 Manual exposureAfter a photomontage has been defined, the montage can be exposed manually. When Manualexposure is started, the following dialog is brought up.The Manual exposure progress dialog.At the top the software indicates the progress of the procedure (in this case no exposures have beenrecorded yet).Underneath the progress bar various values are listed such as the currently available exposure stock,exposure number and exposure time.At bottom right there are two selections (for plate camera only) that allow automatic modification of thefilm text on the plate. If the first is checked, a line is entered in the film text with 'Montage exposure #'where # is replaced by the sequence number of the exposure. If the second is checked a line is enteredwith 'Loc X xxx.xx Y yyy.yy' where xxx.xx and yyy.yy are replaced by the stage x,y location of theexposure. Both options can be used together. Existing film text will be shifted down (if more than 4 lines