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Tecnai on-line help manual -- Options - UT Southwestern

Tecnai on-line help manual -- Options - UT Southwestern

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<str<strong>on</strong>g>Tecnai</str<strong>on</strong>g> <strong>on</strong>-<strong>line</strong> <strong>help</strong> Opti<strong>on</strong>s 38Opti<strong>on</strong>s Software versi<strong>on</strong> 23.6.7 Help menuThe Help menu c<strong>on</strong>tains two items, Show and About. Selecting Show (or pressing F1) will display thisHelp file in the <str<strong>on</strong>g>Tecnai</str<strong>on</strong>g> User Interface. About will display the About TEM Photom<strong>on</strong>tage dialog listing thecurrent versi<strong>on</strong> number of the software. When reporting bugs, always include the versi<strong>on</strong> number in thereport.3.7 C<strong>on</strong>trol opti<strong>on</strong>sThe software supports a range of opti<strong>on</strong>s, defined in the C<strong>on</strong>trol opti<strong>on</strong>s dialog. Opti<strong>on</strong>s are stored asset for each user separately and recalled up<strong>on</strong> restart. Opti<strong>on</strong>s can be saved to file and reloaded.Plate typeThe software supports rapid selecti<strong>on</strong> of exposure sizes from any of the three pre-defined plate cameraformats (the inch size, European cm format and Japanese cm format).The Camera dimensi<strong>on</strong>s c<strong>on</strong>sists of two values, stored separately for each camera, for the horiz<strong>on</strong>taland vertical size of the exposure. In the case of the film cameras, horiz<strong>on</strong>tal is left to right for themicroscope operator (l<strong>on</strong>gest dimensi<strong>on</strong> for plate film), vertical is towards and away from the operator.It is advisable to check the values for the system used. Measure <strong>on</strong> a used negative the l<strong>on</strong>gest(= horiz<strong>on</strong>tal) and shortest (= vertical) exposed area (discounting the plate number). Subtract 2 to 3 mmto account for reproducibility of the plate camera.CCDNot relevant for plate photom<strong>on</strong>tage.OverlapThe overlap between the exposures is the percentage of the horiz<strong>on</strong>tal or vertical axis that must becomm<strong>on</strong> to both exposures to ensure fit of the photom<strong>on</strong>tage without gaps. The overlap can be seteither automatically or at a fixed value. If d<strong>on</strong>e automatically the program will adjust the overlap for themagnificati<strong>on</strong> used (a higher value at higher magnificati<strong>on</strong>, typical values are 10% at 5000x or below,20% at 30kx, 30% at 50kx).Note: It is allowed to define a negative fixed overlap value. In that case the individual exposures will notoverlap but instead be spaced apart. In this way an area can be covered by a regular grid of exposureswithout requiring them to fill the whole area. This functi<strong>on</strong> can be used for example for statisticalanalysis.

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