Tecnai on-line help manual -- Options - UT Southwestern

<strong>Tecnai</strong> on-line help Options 9Options Software version 2These buttons and knobs are assigned in the Options tab of the Low Dose flap-out. Low Dose keepstrack of any other assignment to these buttons and knobs (e.g. on alignment or stigmation). If a button orknob is given another function, its will no longer affect Low Dose (so pressing L1 when the L1 functionhas been changed doesn't switch to Search anymore). As soon as the button or knob has beenreleased, it gets back its Low Dose functionality.When Low Dose is stopped (by pressing the Low Dose button or exiting the <strong>Tecnai</strong> User Interface), thebuttons and knobs get back their original setting.1.1.7 User levels in Low DoseThe Low Dose software supports two user levels, 'user' and 'supervisor'. Supervisor settings (both LowDose settings and calibrations) are stored separately from those of the user. When a new user startsLow Dose for the first time, the settings and calibrations used are those defined by the supervisor.Thereafter the user has set his/her own settings and these will be retrieved when the software is started.The only way thereafter to go back to the supervisor settings is to have the supervisor save settings andcalibrations files and load these files as a user. Low Dose settings and calibrations saved by Factory orService are equivalent to Supervisor settings.1.1.8 SpotscanSpotscan (FP5408/00) is an option for the Low Dose software. It relies on hardware installed in themicroscope PC and microscope and will not function without the proper hardware.The Low Dose version with spotscan supports two modes of exposure:• The normal 'flood-beam' exposure wherein the whole exposure area is illuminated by a broad beamand exposed together.• The spotscan exposure wherein a smaller beam is stepped across the area to be exposed and eachspot is exposed individually.Spotscan settings are defined in the Spotscan tab of the Low Dose Control Panel flap-out.