Tecnai on-line help manual -- Options - UT Southwestern

<strong>Tecnai</strong> on-line help Options 74Options Software version 2By double clicking with the left-hand mouse button on the area covered by the screen, the software isinstructed to change the tilt of the CompuStage by the values corresponding to the point where themouse button was clicked.Convention: The tilt under mouse-button control is such that the point corresponding to the cursorlocation is brought to the center of the diffraction pattern. Note that this tilt is in the opposite direction tothe movement indicated by the tilt axes on the screen.Status barThe status bar lists on the left the current stage position. In the center it displays the major optionschosen for the grid scan. Error messages can be displayed on the right.Border iconsThe border icons appear to behave as the standard Minimize, Maximize and Close buttons. The Closebutton, however, does not truly close the window but hides it. The window is closed only upon closingthe <strong>Tecnai</strong> User Interface.5.6 The menuThe menu consists of five main menus with submenus:• File• Calibrate• Tilt• Focus• Help5.6.1 File menuThe File Menu provides the operations that are concerned with file operations and toolbar display.Load calibrationsSave calibrations,Save calibrations asButtons visibleOpens an existing Smart Tilt calibration file.Saves the Smart Tilt calibration data in a file, Save as allows definition ofdifferent file name.Determines whether the toolbar buttons are visible (menu item checked) ornot.5.6.2 Calibrate menuIn the Calibrate Menu the calibration procedures are found. The calibrations required by Smart Tilt aretwo-fold:• The apparent rotation between the diffraction shift and the viewing screen for the different cameralengths.• The calibration of the diffraction shift (rotation and distance) against the alpha and beta tilts.Both types of calibrations are necessary for proper operation of Smart Tilt. Calibrations can be saved ina file and reloaded. Calibrations are also automatically stored for the user once a procedure has beenfinished successfully.All camera lengthsCurrent camera lengthAlpha tiltBeta tiltCalibration procedure going through all camera lengths.Calibration procedure for the current camera length.Calibration of the alpha tilt against the diffraction shift.Calibration of the beta tilt against the diffraction shift.