official journal of the patent office - Controller General of Patents ...

(12) PATENT APPLICATION PUBLICATION
(19) INDIA (21) Application No.: 1211/MUM/2004 A
(22) Date of filing of Application: 09/11/2004 (43) Publication Date: 09/06/2006
(54) Title of the invention: A NOVEL PROCESS FOR PURIFICATION OF HUMAN GROWTH
HORMONE
(51) International classification : C07K 14/61,
14/35, A61K
38/00
(31) Priority Document No : NIL
(32) Priority Date : NIL
(33) Name of priority country : NIL
(86) International Application No : NIL
and Filing Date : NIL
(87) International Publication No : NIL
(61) Patent of addition to :
Application No
NIL
(62)
Filed on : N.A.
Divisional to Application No : NIL
Filed on : N.A.
(71) Name of Applicant:
USV LTD.
Address of the Applicant:
B.S.D.MARG, GOVANDI, MUMBAI-
400 088, INDIA.
(72) Name of the Inventor:
1. BORBHUIYA MONSUR AHMED
2. RAO YADA MADHAVA
3. RAO LAXMI SRINIVAS
4. MAITI DIPANWITA
5. NIPHADKAR MILIND
PRABHAKAR
6. MISHRA SHRIKANT
Filed U/S 5(2) before The Patents
(Amendment) Act, 2005: NO
(57) Abstract: The present invention relates to a commercial process for purification of human Growth hormone
obtained by DNA recombinant technique using hydrophobic interactive chromatography, efficient to separate
clipped molecules of human growth hormone present as an impurity arising due to clipping molecules of human
growth hormone present as an impurity arising due to clipping positions present on the megamolecules of human
growth hormone from the target human growth hormone molecule using a phenyl column, mixture of aqueous
buffers and organic solvents as an eluant, followed by desalting, lypholization and the invention also relates to the
process wherein the elution of clipped molecules of human growth precedes the elution of target human growth
hormone to obtain hGH of purity>99.5%
Drawing: 12 Sheets.
Total Pages: 34.
Fig. Nil
The Patent Office Journal 09/06/2006 10888