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29 Functional Genomic Approaches for Studies of Mycorrhizal Symbiosis 589<br />

Fig. 7. Histogram showing<br />

clustering of genes from<br />

macroarray analysis based<br />

on levels of expression. The<br />

interaction (72 h)-related<br />

expression is shown on the<br />

x-axis and the number of<br />

genes that are upregulated<br />

at a given expression ratio<br />

is shown on the y-axis.Only<br />

genes that are upregulated<br />

are shown from the<br />

macroarray in Fig. 6A. For<br />

identification of some of<br />

the genes upregulated by<br />

interaction see Table 2<br />

Fig. 8. Scatter plot analysis of interaction-specific genes between free-living Laccaria<br />

bicolor and L. bicolor interacted 72 h with Pinus resinosa seedling roots. For each gene,<br />

transcript levels were calculated for the free-living mycelium and the mycelium that<br />

interacted with red pine seedling root signals. Solid lines indicate an expression level of<br />

onefold or above the free-living mycelium, dashed lines 2.5–6-fold increase and dotted<br />

line eightfold or higher levels of expression. Only nonredundant clones are represented<br />

in the plot. Selected clones that showed significant differential expression are highlighted

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