plant surface microbiology.pdf

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XXII Contents 6.5 CMEIAS v. 3.0: In Situ Geostatistical Analysis of Root Colonization by Pioneer Rhizobacteria . . . . . . . 540 6.6 CMEIAS v. 3.0: Quantitative Autecological Biogeography of the Rhizobium–Rice Association . . . . . . . . . . . . . . 541 6.7 CMEIAS v. 3.0: Spatial Scale Analysis of in Situ Quorum Sensing by Root-Colonizing Bacteria . . . . . . . . 543 7 Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . 544 References and Selected Reading . . . . . . . . . . . . . . . . . . . . . 544 28 Analysis of Microbial Population Genetics . . . . . . . . . . 551 Emanuele G. Biondi, Alessio Mengoni and Marco Bazzicalupo 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . 551 2 Materials for RAPD, AFLP and ITS . . . . . . . . . . . . . . 552 3 RAPD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 553 4 AFLP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 556 5 ITS-RFLP Analysis . . . . . . . . . . . . . . . . . . . . . . . 559 6 Statistical analysis . . . . . . . . . . . . . . . . . . . . . . . . 561 7 Concluding Remarks . . . . . . . . . . . . . . . . . . . . . . 563 References and Selected Reading . . . . . . . . . . . . . . . . . . . . . 564 29 Functional Genomic Approaches for Studies of Mycorrhizal Symbiosis . . . . . . . . . . . . . . . . . . . 567 Gopi K. Podila and Luisa Lanfranco 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . 567 2 Material and Methods . . . . . . . . . . . . . . . . . . . . . . 568 2.1 Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . 568 2.2 Biological Material . . . . . . . . . . . . . . . . . . . . . . . 569 2.3 RNA Extraction . . . . . . . . . . . . . . . . . . . . . . . . . 569 3 RNA Quantification . . . . . . . . . . . . . . . . . . . . . . . 570 3.1 Construction of a cDNA Library . . . . . . . . . . . . . . . . 570 4 Conversion Protocol . . . . . . . . . . . . . . . . . . . . . . 577 4.1 Evaluation of the Quality of the cDNA Library . . . . . . . . 577 5 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . 578 6 Sequencing Strategies . . . . . . . . . . . . . . . . . . . . . . 578 6.1 Data Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . 579 6.2 Sequence Homology Comparisons . . . . . . . . . . . . . . 579
Page 2: PLANT SURFACE MICROBIOLOGY
Page 6: Professor Dr. Ajit Varma Director A
Page 10: VI ology. The basic concepts in pla
Page 14: VIII Contents 6.1 Abiotic Factors .
Page 18: X Section B Contents 7 The Function
Page 22: XII 3 Impact of Genetically Modifie
Page 26: XIV 4.9 Arabidopsis thaliana . . .
Page 30: XVI Contents 4.1 Diversity of Arbus
Page 34: XVIII 6.2 Role and Properties of Gl
Page 38: XX Contents 4.1 Isolated Cuticles a
Page 44: Contents XXIII 6.3 Examples of Expr
Page 48: XXVI Contributors Belanger, Faith D
Page 52: XXVIII Contributors Kottke, Ingrid
Page 56: XXX Contributors Pukall, Rüdiger D
Page 60: 1 The State of the Art Ajit Varma,
Page 64: 1 The State of the Art 3 microsymbi
Page 68: 1 The State of the Art 5 symbiotic
Page 72: 1 The State of the Art 7 responses
Page 76: 1 The State of the Art 9 great adva
Page 80: 1 The State of the Art 11 erature i
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18 Thomas F.C. Chin-A-Woeng and Ben
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20 Table 1. Reporter genes commonly
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3 Methanogenic Microbial Communitie
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3 Methaogenic Microbial Communities
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4 Role of Functional Groups of Micr
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Population 1 Population 2 Populatio
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4 Microorganisms on the Rhizosphere
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6 Functional Groups of Microrganism
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72 Ramesh Chander Kuhad et al. need
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74 Ramesh Chander Kuhad et al. meas
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76 Ramesh Chander Kuhad et al. 3 Ro
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78 Ramesh Chander Kuhad et al. root
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80 Ramesh Chander Kuhad et al. domi
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82 Ramesh Chander Kuhad et al. from
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84 Ramesh Chander Kuhad et al. 3.4
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86 Ramesh Chander Kuhad et al. cell
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88 Ramesh Chander Kuhad et al. of b
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90 Ramesh Chander Kuhad et al. in s
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92 Ramesh Chander Kuhad et al. Berc
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94 Ramesh Chander Kuhad et al. Hawk
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96 Ramesh Chander Kuhad et al. Pros
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98 Ramesh Chander Kuhad et al. Vier
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100 Dietrich Werner Fig. 1. Notch t
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102 Dietrich Werner 2.1 Phenylpropa
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104 Dietrich Werner 2.2 Metabolizat
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106 Dietrich Werner equol, genistei
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108 Dietrich Werner Table 2. Modifi
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110 Dietrich Werner 3.3 Lipopolysac
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112 Dietrich Werner There is increa
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114 Dietrich Werner antimicrobial f
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116 Dietrich Werner Hofmann K, Hein
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118 Dietrich Werner Schröder O, Wa
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7 The Functional Groups of Micro-or
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8 The Use of ACC Deaminase-Containi
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wood-rotting fungi, were shown to i
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8 ACC Deaminase-Containing Plant Gr
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9 Interactions Between Epiphyllic M
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However, at the moment, the mechani
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10 Developmental Interactions Betwe
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10 Development Interactions Between
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11 Interactions of Microbes with Ge
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GMP species, Group(s) of organisms
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a single copy gene of A. muscaria (
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12 Interaction Between Soil Bacteri
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Table 1. Protein features and data
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13 The Surface of Ectomycorrhizal R
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mrc rc rc a b c d 13 Root Surface i
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ccw cc rccw cc rccw ccw ph rccw rcc
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cc cc cc sl cc rcc 13 Root Surface
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13 Root Surface in Ectomycorrhizas
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ccw cc hy sl rccw hy hy 7 Hartig Ne
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9 Conclusions 13 Root Surface in Ec
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13 Root Surface in Ectomycorrhizas
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14 Cellular Ustilaginomycete - Plan
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5 Cellular Interactions 14 Cellular
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Fig. 3. Local interaction zone with
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14 Cellular Ustilaginomycete - Plan
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6 Conclusions 14 Cellular Ustilagin
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15 Interaction of Piriformospora in
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268 Suberkropp 1990; Bauer et al. 1
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270 Robert Bauer and Franz Oberwink
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17 Fungal Endophytes Sita R. Ghimir
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17 Fungal Endophytes 283 increase d
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comprises dipping samples in 96 % e
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with saprobic fungi. Host exclusivi
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17 Fungal Endophytes 289 Carroll G
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17 Fungal Endophytes 291 Mills PR,
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18 Mycorrhizal Development and Cyto
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2.2 Expression of Actin-Encoding Ge
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6 Actin Binding Proteins 18 Mycorrh
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etween the function of cell cycle a
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hiza. Cells with well-preserved cyt
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332 M. Zakaria Solaiman and Lynette
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20 Mycorrhizal Fungi and Plant Grow
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een described as a plant-growth-pro
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374 Uwe Nehls 2 Trehalose Utilizati
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376 Uwe Nehls mon to ectomycorrhiza
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378 Uwe Nehls monosaccharide transp
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380 Uwe Nehls Fig. 2. Spatial distr
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382 Uwe Nehls quite untypical for f
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384 Uwe Nehls the utilization of al
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386 Uwe Nehls have been described.
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388 Uwe Nehls script profiling duri
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390 Uwe Nehls Scheller E (1996) Ami
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22 Nitrogen Transport and Metabolis
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432 Thomas F.C. Chin-A-Woeng et al.
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450 Anton Hartmann et al. The rhizo
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452 Table 1. Phylogenetic oligonucl
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454 Anton Hartmann et al. B D
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456 Anton Hartmann et al. root surf
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458 Anton Hartmann et al. (see abov
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460 Anton Hartmann et al. to 13, 26
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462 Anton Hartmann et al. root surf
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464 Anton Hartmann et al. media are
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466 Anton Hartmann et al. Gorlach K
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468 Anton Hartmann et al. Poindexte
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25 Methods for Analysing the Intera
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25 Analysing Interactions between M
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into the nutrition solution inside
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An example for the change in water
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490 Donna M. Penrose and Bernard R.
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494 Absorbance at 540 nm 1.6 1.4 1.
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504 Frank B. Dazzo electron, and fi
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506 Frank B. Dazzo the microsymbion
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508 Frank B. Dazzo degrade the bact
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510 Frank B. Dazzo Fig. 4. Phase co
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512 Frank B. Dazzo Fig. 5. Symbiont
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514 Frank B. Dazzo microscopy. The
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516 Frank B. Dazzo the rhizobial ex
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518 Frank B. Dazzo In contrast, qua
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520 Frank B. Dazzo biological activ
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522 Frank B. Dazzo NBD-labeled CLOS
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524 Frank B. Dazzo cated that the s
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526 Frank B. Dazzo to introduce pol
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528 Frank B. Dazzo response is less
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530 Frank B. Dazzo mizing the gyror
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532 Frank B. Dazzo including its re
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534 Frank B. Dazzo Table 1. Quantit
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536 Frank B. Dazzo and eukaryotic c
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538 Frank B. Dazzo Table 2. In situ
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540 Frank B. Dazzo involvement of t
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542 Frank B. Dazzo Fig. 16. Geostat
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544 Frank B. Dazzo the power of geo
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546 Frank B. Dazzo Dazzo FB, Hollin
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548 Frank B. Dazzo erney MJ, Stetze
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550 Frank B. Dazzo van Workum WAT,
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552 Emanuele G. Biondi 2 Materials
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554 Emanuele G. Biondi eral species
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556 - Low intensity of the bands: i
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558 Emanuele G. Biondi CCA ATT CT-3
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560 Emanuele G. Biondi Experimental
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562 Emanuele G. Biondi two strains
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564 Emanuele G. Biondi References a
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29 Functional Genomic Approaches fo
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30 Axenic Culture of Symbiotic Fung
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9 Phosphatic Nutrients 30 Axenic Cu
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Modified aspergillus medium (Varma
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616 Subject Index Antifungal activi
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618 Subject Index Cryosection 317 C
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620 Subject Index Fusarium sp. 73,
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622 Subject Index Leaf surface colo
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624 Subject Index Penicillium sp. 7
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626 Subject Index Salmon sperm DNA
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628 Subject Index Y Yellow fluoresc
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