plant surface microbiology.pdf

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Giang Huong Pham et al.
Table 3. Comparative linear growth of P. indica on different
solidified agar media. The data represent an average
colony diameter of five replicates, measured after
5 days of incubation
Media Linear measurement of the growth (cm)
MMN 4.2±0.05
M4N 2.8±0.09
PDA 3.5±0.11
aspergillus 3.6±0.15
Table 4. Change of pH of the aspergillus broth medium incubated with P. indica
Medium conditions (pH) Incubation days
0 3 5 7 10
Unbuffered 6.5 6.0 5.7 5.1 4.4
Buffered 6.5 6.5 6.5 6.5 6.3
Initial pH was adjusted to 6.5. 25–100 mM MES was used as buffering agent
Table 5. End pH and biomass of P. indica grown on minimal
aspergillus broth medium containing different sugars (each 1 %
w/v)
Sugars End pH Biomass (mg/10 ml)
Control (no addition) 5.18 7.5
Glucose 4.39 10.0
Fructose 5.25 8.0
Maltose 4.46 12.0
Rhamnose 4.60 10.3
Mannose 4.30 9.5
Lactose 4.44 10.0
Sucrose 4.39 10.0
Xylose 4.31 11.0
Arabinose 4.28 10.0
Raffinose 4.43 9.0
One agar disc (1 cm in diameter loaded with hyphae and
chlamydospores) was transferred to individual test tubes containing
10 ml minimal broth. Sterile sugar solution (microsyringe-filtered,
0.22 mm Schleicher & Schuell) was included. Incubation
was done under constant shaking conditions (GFL, 3026,
Germany) for 7 days at 25 °C. Fungal biomass was removed and
end pH was measured