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606<br />

phatase in addition to the constitutive one observed in the enzyme staining of<br />

the native polyacrylamide gels. The significance of these enzymes in the phosphate<br />

transport needs to be further substantiated by the studies on the <strong>plant</strong><br />

roots colonized with P. indica.<br />

10 Composition of Media<br />

a Aspergillus (Kaefer 1977)<br />

Composition (g/l)<br />

Glucose 20.0<br />

Peptone 2.0<br />

Yeast extract 1.0<br />

Casein hydrolysate 1.0<br />

Vitamin stock solution 1.0 ml<br />

Macro-elements from stock 50.0 ml<br />

Micro-elements from stock 2.5 ml<br />

Agar 10.0<br />

CaCl2 0.1 M 1.0 ml<br />

FeCl3 0.1 M 1.0 ml<br />

pH<br />

Macro-elements<br />

6.5<br />

(Major elements) Stock (g/l)<br />

NaNO3 120.0<br />

KCl 10.4<br />

MgSO .<br />

4 7H2O 10.4<br />

KH2PO4 Micro-elements<br />

30.4<br />

Trace elements Stock (g/l)<br />

ZnSO .<br />

4 7H2O 22.0<br />

H 3 BO 3<br />

Giang Huong Pham et al.<br />

11.0<br />

MnCl 2 . 4H2O 5.0<br />

FeSO 4 . 7H2O 5.0<br />

CoCl 2 . 6H2O 1.6<br />

CuSO 4 . 5H2O 1.6<br />

(NH 4) 6 Mo 7O 27 4H 2O 1.1<br />

Na 2EDTA 50.0<br />

Vitamins % (w/v)<br />

Biotin 0.05<br />

Nicotinamide 0.5<br />

Pyridoxal phosphate 0.1<br />

Amino benzoic acid 0.1<br />

Riboflavin 0.25<br />

The pH was adjusted to 6.5 with 1 N HCl. All the stocks were stored at 4 °C<br />

except the vitamins which were stored at –20 °C

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