plant surface microbiology.pdf

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6.2 Role and Properties of Glutamine Synthetase . . . . . . . . 413
6.3 Role and Properties of Glutamate Synthase . . . . . . . . . . 415
7 Amino Acid Metabolism . . . . . . . . . . . . . . . . . . . . 417
7.1 Utilization of Proteins by Ectomycorrhizal Fungi
and Ectomycorrhizas . . . . . . . . . . . . . . . . . . . . . . 417
7.2 Amino Acids Used as Nitrogen and Carbon Sources . . . . . 418
8 Conclusion and Future Prospects . . . . . . . . . . . . . . . 419
References and Selected Reading . . . . . . . . . . . . . . . . . . . . . 421
Section E
Contents
23 Visualisation of Rhizosphere Interactions
of Pseudomonas and Bacillus Biocontrol Strains . . . . . . 431
Thomas F.C. Chin-A-Woeng, Anastasia L. Lagopodi,
Ine H.M. Mulders, Guido V. Bloemberg
and Ben J.J. Lugtenberg
1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . 431
2 Tomato Foot and Root Rot and the Need
for Biological Control . . . . . . . . . . . . . . . . . . . . . . 431
3 Selection of Antagonistic Strains . . . . . . . . . . . . . . . 432
3.1 Selection of Antagonistic Pseudomonas and Bacillus sp. . . . 432
3.2 In Vitro Antifungal Activity Test . . . . . . . . . . . . . . . . 434
4 In Vivo Biocontrol Assays . . . . . . . . . . . . . . . . . . . . 434
4.1 Fusarium oxysporum—Tomato Biocontrol Assay
in a Potting Soil System . . . . . . . . . . . . . . . . . . . . . 434
4.2 Gnotobiotic Fusarium oxysporum–Pythium ultimum
and Rhizoctonia solani–Tomato Bioassays . . . . . . . . . . 435
5 Microscope Analysis of Infection and Biocontrol . . . . . . 435
5.1 Marking Fungi with Autofluorescent Proteins . . . . . . . . 437
5.2 Marking Rhizosphere Bacteria with Autofluorescent
Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 438
5.3 Confocal Laser Scanning Microscopy
of Rhizosphere Interactions . . . . . . . . . . . . . . . . . . 442
6 Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . 443
References and Selected Reading . . . . . . . . . . . . . . . . . . . . . 443